{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T06:32:10Z","timestamp":1780641130422,"version":"3.54.1"},"reference-count":75,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,2,24]],"date-time":"2023-02-24T00:00:00Z","timestamp":1677196800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Fraxinus xanthoxyloides<\/jats:italic> is a perennial shrub belonging to family Oleaceae, traditionally used for malaria, jaundice, pneumonia, inflammation, and rheumatism. Our study is aimed to assess the total phenolics (TPC), flavonoids (TFC), terpenoids contents (TTC) and antioxidant profiling of <jats:italic>F. xanthoxyloides<\/jats:italic> methanol bark extract (FXBM) and its fractions, hexane, chloroform, ethyl acetate and aqueous, along with high\u2010performance liquid chromatography with diode\u2010array detection (HPLC\u2010DAD). Further, the antioxidant and pulmonary protective potential was explored against carbon tetrachloride (CCl<jats:sub>4<\/jats:sub>)\u2010induced CCl4\u2010induced pulmonary tissue damage in rats. The highest TPC, TFC and TTC were found in FXBM (133.29\u00b14.19\u2005mg\/g), ethyl acetate fraction (279.55\u00b110.35\u2005mg\/g), and chloroform fraction (0.79\u00b10.06\u2005mg\/g), respectively. The most potent antioxidant capacity was depicted by FXBM (29.21\u00b12.40\u2005\u03bcg\/mg) and ethyl acetate fraction (91.16\u00b15.51\u2005\u03bcg\/mg). The HPLC\u2010DAD analysis revealed the predominance of gallic, chlorogenic, vanillic and ferulic acid in FXBM. The administration of CCl<jats:sub>4<\/jats:sub> induced oxidative stress, suppressed antioxidant enzymes\u2032 activities including catalase, peroxidase, superoxide dismutase, glutathione peroxidase, glutathione\u2010S\u2010transferase, and glutathione reductase. Further, it increased thiobarbituric acid reactive substances (TBARS) and H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> levels, induced DNA injuries and reduced the total protein and glutathione content in lung tissues. The treatment of rats with FXBM restored these biochemical parameters to the normal level. Moreover, the histopathological studies of lung tissues demonstrated that FXBM protected rats\u2032 lung tissues from oxidative damage restoring normal lung functions. Thus, <jats:italic>F. xanthoxyloides<\/jats:italic> bark extract is recommended as adjuvant therapy as protective agent for patients with lung disorders.<\/jats:p>","DOI":"10.1002\/cbdv.202200755","type":"journal-article","created":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T12:53:13Z","timestamp":1675255993000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Antioxidant and Pulmonary Protective Potential of <i>Fraxinus xanthoxyloides<\/i> Bark Extract against CCl<sub>4<\/sub>\u2010Induced Toxicity in Rats"],"prefix":"10.1002","volume":"20","author":[{"given":"Tahira","family":"Younis","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Biotechnology The Women University Multan  60000 Multan Pakistan"},{"name":"Department of Biochemistry Quaid-i-Azam University  45320 Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faiza","family":"Jabeen","sequence":"additional","affiliation":[{"name":"Department of Zoology University of Education Bank road campus  54000 Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Hussain","sequence":"additional","affiliation":[{"name":"Department of Zoology University of the Punjab  54590 Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bilal","family":"Rasool","sequence":"additional","affiliation":[{"name":"Department of Zoology Government College University  38000 Faisalabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Raza Ishaq","sequence":"additional","affiliation":[{"name":"Department of Zoology Government College University  38000 Faisalabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atif","family":"Nawaz","sequence":"additional","affiliation":[{"name":"Department of Zoology Government College University  38000 Faisalabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4252-7647","authenticated-orcid":false,"given":"Heba A. 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