{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T00:04:04Z","timestamp":1778630644257,"version":"3.51.4"},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,3,10]],"date-time":"2023-03-10T00:00:00Z","timestamp":1678406400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32060074"],"award-info":[{"award-number":["32060074"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31660384"],"award-info":[{"award-number":["31660384"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>As a traditional Chinese medicine, <jats:italic>Rhododendron molle<\/jats:italic> G. Don has a long history of treating rheumatoid arthritis. In this study, RAW 264.7 cells induced by lipopolysaccharide (LPS) were established as cell inflammatory model to evaluate the anti\u2010inflammatory activity of chloroform extract from <jats:italic>R. molle<\/jats:italic> leaves (CERL), ethyl acetate extract from <jats:italic>R. molle<\/jats:italic> leaves (EERL) and butanol extract from <jats:italic>R. molle<\/jats:italic> leaves (BERL) and analyze the potential anti\u2010inflammatory components of <jats:italic>R. molle<\/jats:italic>. Potential anti\u2010inflammatory components analysis of CERL were performed by HPLC and UHPLC\u2010Q\u2010TOF\u2010MS. Prediction of potential anti\u2010inflammatory components by molecular docking experiments. Compared with negative control group, 25\u2005\u03bcg\/mL CERL could reduce the release level of NO by 62\u2009%, and the mRNA expression levels of <jats:italic>COX\u20102<\/jats:italic>, <jats:italic>IL\u20106<\/jats:italic>, <jats:italic>IL\u20101\u03b2<\/jats:italic> and <jats:italic>TNF\u2010\u03b1<\/jats:italic> were reduced by 69.74\u2009%, 86.25\u2009%, 77.94\u2009% and 56.80\u2009%, respectively. Western\u2010Blot showed similar results. CERL, EERL and BERL exerted their inhibitory activity in dose\u2010dependent manner. All results showed that the higher the concentration, the better the anti\u2010inflammatory activity. CERL showed the best inhibitory activity, the second was EERL, and then was BERL. 21 terpenoids and 4 flavonoids were identified in CERL by UHPLC\u2010Q\u2010TOF\u2010MS. Molecular docking results showed that triterpenoids in CERL had better interaction with target proteins (TNF\u2010\u03b1, IL\u20101\u03b2). It indicated that triterpenoids may be potential anti\u2010inflammatory components of <jats:italic>R. molle<\/jats:italic> leaves. This study explored the anti\u2010inflammatory activities of CERL, EERL, BERL, which laid a foundation for further promoting the clinical application of <jats:italic>R. molle<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/cbdv.202201132","type":"journal-article","created":{"date-parts":[[2023,2,27]],"date-time":"2023-02-27T02:58:35Z","timestamp":1677466715000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Potential Anti\u2010Inflammatory Components of <i>Rhododendron molle<\/i> G. Don Leaf Extracts in LPS\u2010Induced RAW 264.7"],"prefix":"10.1002","volume":"20","author":[{"given":"Yuqiang","family":"Luo","sequence":"first","affiliation":[{"name":"College of life science Jiangxi Normal University  Nanchang 330022 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Decai","family":"Wu","sequence":"additional","affiliation":[{"name":"College of life science Jiangxi Normal University  Nanchang 330022 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luye","family":"Zong","sequence":"additional","affiliation":[{"name":"College of life science Jiangxi Normal University  Nanchang 330022 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Shen","sequence":"additional","affiliation":[{"name":"College of life science Jiangxi Normal University  Nanchang 330022 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liangfang","family":"Dai","sequence":"additional","affiliation":[{"name":"College of life science Jiangxi Normal University  Nanchang 330022 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6466-7144","authenticated-orcid":false,"given":"Xiangdong","family":"Luo","sequence":"additional","affiliation":[{"name":"College of life science Jiangxi Normal University  Nanchang 330022 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,3,10]]},"reference":[{"key":"e_1_2_10_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2014.10.002"},{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2017.04.003"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2015.06.004"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-019-0572-1"},{"key":"e_1_2_10_5_1","first-page":"1359","article-title":"\u2018Specific and selective induction of miR-124 in immune cells by the quinoline ABX464: a transformative therapy for inflammatory diseases\u2019","volume":"26","author":"Tazi J.","year":"2020","journal-title":"Drug Discovery Today."},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2020.01.009"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1753-4887.2007.tb00352.x"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.05.055"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9040880"},{"key":"e_1_2_10_10_1","first-page":"143","article-title":"\u2018A comprehensive review of non-steroidal anti-inflammatory drug use in the elderly\u2019","volume":"9","author":"Wongrakpanich S.","year":"2018","journal-title":"Aging"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jnatprod.5b01055"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.8664"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2010.10.053"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00296-010-1370-0"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1875-5364(18)30073-6"},{"key":"e_1_2_10_16_1","first-page":"1350","article-title":"\u2018Research progress on chemical constituents of Rhododendron molle and its pharmacological activities\u2019","volume":"51","author":"Xue Q. 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