{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,7]],"date-time":"2026-06-07T04:20:58Z","timestamp":1780806058846,"version":"3.54.1"},"reference-count":48,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,5,2]],"date-time":"2023-05-02T00:00:00Z","timestamp":1682985600000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Our previous study has exhibited that one kind of <jats:italic>Zanthoxylum bungeanum<\/jats:italic> seed oil (ZSO), extracted from <jats:italic>Zanthoxylum bungeanum<\/jats:italic> seed, had inhibitory effects on osteoclastogenesis. However, the anti\u2010osteoclastogenesis activities of different kinds of ZSO are scarcely reported. Since inflammation is related to bone loss and osteoporosis, in this study, three kinds of ZSO, <jats:italic>Zanthoxylum schinifolium<\/jats:italic> Siebold et Zucc seed oil (ZSSO), <jats:italic>Zanthoxylum armatum<\/jats:italic> DC. seed oil (ZDSO) and <jats:italic>Zanthoxylum bungeanum<\/jats:italic> maximum seed oil (ZBSO), were obtained with Soxhlet extraction and their fatty acid constituents were detected by GC\u2010FID. RAW264.7 macrophages induced by lipopolysaccharide (LPS) were used to evaluate the inhibitory effects of three kinds of ZSO on inflammation via detecting the expression levels of inflammatory factors by RT\u2010qPCR. Moreover, RANKL\u2010induced osteoclastogenesis was applied to demonstrate the anti\u2010osteoclastogenesis activities of them through tartrate\u2010resistant acid phosphatase (TRAP) staining and RT\u2010qPCR. The GC\u2010FID results exhibited that the highest constituent in ZSSO and ZDSO was oleic acid (OA) and palmitoleic acid (PLA), respectively. While linoleic acid (LA) and \u03b1\u2010Linolenic acid (ALA) in ZBSO were dominant. At the concentration of 0.5\u2005\u03bcL\/mL, all three kinds of ZSO could decrease the expression levels of inducible NO synthase (iNOS), cyclooxygenase\u20102 (COX\u20102), tumor necrosis factor\u2010\u03b1 (TNF\u2010\u03b1), interleukin\u20106 (IL\u20106), interleukin\u20101 beta (IL\u20101\u03b2) in LPS\u2010induced macrophages. At the concentration of 0.25\u2005\u03bcL\/mL, only ZSSO could decrease the expression levels of iNOS and COX\u20102, which implied the inhibitory effects of ZSSO were stronger than other ZSOs. The number of RANKL\u2010induced osteoclasts and the expressions of nuclear factor kappa\u2010B (NF\u2010\u03baB), TNF\u2010\u03b1 and IL\u20106 in the cells were decreased after being treated with ZSOs at the concentration of 0.5\u2005\u03bcL\/mL, while the number of RANKL\u2010induced osteoclasts after treated with ZBSO were less than those treated with other ZSOs, this indicated that the anti\u2010osteoclastogenesis effect of ZBSO were stronger than other ZSOs. In conclusion, the fatty acid compositions of three major kinds of ZSO were compared and the content of unsaturated fatty acids especially \u03c9\u20103 polyunsaturated fatty acids in ZBSO were the highest among them. All ZSOs tested had anti\u2010inflammatory and anti\u2010osteoclastogenesis activities. And their anti\u2010osteoclastogenesis effects might be related to the suppression of the NF\u2010\u03baB pathway.<\/jats:p>","DOI":"10.1002\/cbdv.202201157","type":"journal-article","created":{"date-parts":[[2023,4,8]],"date-time":"2023-04-08T09:44:03Z","timestamp":1680947043000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Anti\u2010Inflammatory and Anti\u2010Osteoclastogenesis Activities of Different Kinds of <i>Zanthoxylum bungeanum<\/i> Seed Oil <i>in Vitro<\/i>"],"prefix":"10.1002","volume":"20","author":[{"given":"Baochao","family":"Zhang","sequence":"first","affiliation":[{"name":"West China School of Public Health and West China Fourth Hospital Sichuan University  Renmin South Road Chengdu 610041 China"},{"name":"Food Safety Monitoring and Risk Assessment Key Laboratory of Sichuan Province Department of Public Health Laboratory Sciences West China School of Public Health Sichuan University  Renmin South Road Chengdu 610041 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuhan","family":"Luo","sequence":"additional","affiliation":[{"name":"West China School of Public Health and West China Fourth Hospital Sichuan University  Renmin South Road Chengdu 610041 China"},{"name":"Food Safety Monitoring and Risk Assessment Key Laboratory of Sichuan Province Department of Public Health Laboratory Sciences West China School of Public Health Sichuan University  Renmin South Road Chengdu 610041 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanshan","family":"Wu","sequence":"additional","affiliation":[{"name":"West China School of Public Health and West China Fourth Hospital Sichuan University  Renmin South Road Chengdu 610041 China"},{"name":"Food Safety Monitoring and Risk Assessment Key Laboratory of Sichuan Province Department of Public Health Laboratory Sciences West China School of Public Health Sichuan University  Renmin South Road Chengdu 610041 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Healthcare-associated Infection Management The Affiliated Hospital of Guizhou Medical University  Guiyi Road Guiyang 550004 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangting","family":"He","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Chengdu Second People's Hospital  Qingyun South Road Chengdu 610041 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruocheng","family":"Luo","sequence":"additional","affiliation":[{"name":"West China School of Public Health and West China Fourth Hospital Sichuan University  Renmin South Road Chengdu 610041 China"},{"name":"Food Safety Monitoring and Risk Assessment Key Laboratory of Sichuan Province Department of Public Health Laboratory Sciences West China School of Public Health Sichuan University  Renmin South Road Chengdu 610041 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yating","family":"Wu","sequence":"additional","affiliation":[{"name":"West China School of Public Health and West China Fourth Hospital Sichuan University  Renmin South Road Chengdu 610041 China"},{"name":"Food Safety Monitoring and Risk Assessment Key Laboratory of Sichuan Province Department of Public Health Laboratory Sciences West China School of Public Health Sichuan University  Renmin South Road Chengdu 610041 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meiling","family":"Pan","sequence":"additional","affiliation":[{"name":"West China School of Public Health and West China Fourth Hospital Sichuan University  Renmin South Road Chengdu 610041 China"},{"name":"Food Safety Monitoring and Risk Assessment Key Laboratory of Sichuan Province Department of Public Health Laboratory Sciences West China School of Public Health Sichuan University  Renmin South Road Chengdu 610041 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Yong","sequence":"additional","affiliation":[{"name":"Sichuan Center for Disease Control and Prevention  Zhongxue Road Chengdu 610041 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0694-7783","authenticated-orcid":false,"given":"Xiaofang","family":"Pei","sequence":"additional","affiliation":[{"name":"West China School of Public Health and West China Fourth Hospital Sichuan University  Renmin South Road Chengdu 610041 China"},{"name":"Food Safety Monitoring and Risk Assessment Key Laboratory of Sichuan Province Department of Public Health Laboratory Sciences West China School of Public Health Sichuan University  Renmin South Road Chengdu 610041 China"},{"name":"West China-PUMC C. 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