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The prognosis of TNBC is poor because of its high distant metastasis rate. Triptolide is a type of diterpene trioxide natural compound with potential anti\u2010tumor activities. This study explored the metastatic inhibitory effect of triptolide on MDA\u2010MB\u2010231 cells and its underlying mechanism. Triptolide suppressed cell proliferation and induced cell apoptosis in a time\u2010 and dose\u2010dependent manner. Low doses of triptolide (0\u20108\u2005nM) reduced the migration and invasion capabilities of MDA\u2010MB\u2010231 cells. Triptolide decreased ROCK1, p\u2010Akt, N\u2010cadherin, vimentin and MMP\u20109 expressions, but increased PTEN and E\u2010cadherin expressions on protein and mRNA levels. Furthermore, the down\u2010regulation of ROCK1 expression in MDA\u2010MB\u2010231 cells after being treated by triptolide could be rescued by ROCK1 specific inhibitor Y27632. Molecular docking showed that triptolide and Y27632 shared the same active center of ROCK1 protein. This article\u2018s findings taken together showed that ROCK1 is the primary target of triptolide, which can cause cell apoptosis and inhibit the epithelial\u2010mesenchymal transition of MDA\u2010MB\u2010231 cells.<\/jats:p>","DOI":"10.1002\/cbdv.202300399","type":"journal-article","created":{"date-parts":[[2023,11,1]],"date-time":"2023-11-01T18:38:27Z","timestamp":1698863907000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Triptolide Reduces MDA\u2010MB\u2010231 Cell Metastasis by Attenuating Epithelial\u2010Mesenchymal Transition through the ROCK\/PTEN\/Akt Axis"],"prefix":"10.1002","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6718-8501","authenticated-orcid":false,"given":"Qinhang","family":"Wu","sequence":"first","affiliation":[{"name":"School of Pharmacy Nanjing University of Chinese Medicine  Nanjing 210023 P.R. 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