{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T16:44:20Z","timestamp":1784825060178,"version":"3.55.0"},"reference-count":46,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,8,4]],"date-time":"2023-08-04T00:00:00Z","timestamp":1691107200000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Olive leaf extract is a valuable source of phenolic compounds; primarily, oleuropein (major component) and rutin. This natural olive leaf extract has potential use as a therapeutic agent for cancer treatment. However, its clinical application is hindered by poor pharmacokinetics and low stability. To overcome these limitations, this study aimed to enhance the anticancer activity and stability of oleuropein and rutin by loading them into PEGylated Nano\u2010phytosomes. The developed PEGylated Nano\u2010phytosomes exhibited favorable characteristics in terms of size, charge, and stability. Notably, the anticolonic cancer activity of the Pegylated Nano\u2010phytosomes loaded with oleuropein (IC50=0.14\u2005\u03bcM) and rutin (IC50=0.44\u2005\u03bcM) surpassed that of pure oleuropein and rutin alone. This outcome highlights the advantageous impact of Nano\u2010phytosomes to augment the anticancer potential of oleuropein and rutin. These results present a promising pathway for the future development of oleuropein and rutin Nano\u2010phytosomes as effective options for passive tumor\u2010targeted therapy, given their improved stability and efficacy.<\/jats:p>","DOI":"10.1002\/cbdv.202300534","type":"journal-article","created":{"date-parts":[[2023,7,27]],"date-time":"2023-07-27T13:19:19Z","timestamp":1690463959000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Development of Pegylated Nano\u2010Phytosome Formulation with Oleuropein and Rutin to Compare Anti\u2010Colonic Cancer Activity with Olea Europaea Leaves Extract"],"prefix":"10.1002","volume":"20","author":[{"given":"Tabarek H.","family":"Mahmood","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy Pharmacological and Diagnostic Research Center Al-Ahliyya Amman University  Amman Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Al\u2010Samydai","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy Pharmacological and Diagnostic Research Center Al-Ahliyya Amman University  Amman Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mazen Al","family":"Sulaibi","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy Pharmacological and Diagnostic Research Center Al-Ahliyya Amman University  Amman Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moath","family":"Alqaraleh","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy Pharmacological and Diagnostic Research Center Al-Ahliyya Amman University  Amman Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anas Ibrahim","family":"Abed","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy Pharmacological and Diagnostic Research Center Al-Ahliyya Amman University  Amman Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naeem","family":"Shalan","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy Pharmacological and Diagnostic Research Center Al-Ahliyya Amman University  Amman Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alaa","family":"Alsanabrah","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy Pharmacological and Diagnostic Research Center Al-Ahliyya Amman University  Amman Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shrouq Taiseer","family":"Alsotari","sequence":"additional","affiliation":[{"name":"Cell Therapy Center The University of Jordan  Amman 11942 Jordan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5916-5879","authenticated-orcid":false,"given":"Hamdi","family":"Nsairat","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy Pharmacological and Diagnostic Research Center Al-Ahliyya Amman University  Amman Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walhan","family":"Alshaer","sequence":"additional","affiliation":[{"name":"Cell Therapy Center The University of Jordan  Amman 11942 Jordan."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,8,4]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers15030993"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.2147\/CMAR.S330448"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu11010164"},{"key":"e_1_2_8_4_1","first-page":"47","author":"Vishvakrama P.","year":"2014","journal-title":"J. Drug Delivery and Therapeutics"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu11081776"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10101577"},{"key":"e_1_2_8_7_1","first-page":"2","volume":"2017","author":"Tresserra-Rimbau A.","year":"2017","journal-title":"Hindawi"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26134069"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.7920"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.btre.2019.e00347"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/life12081140"},{"key":"e_1_2_8_12_1","first-page":"263","volume":"56","author":"Rahiman S.","year":"2019","journal-title":"Indian J. Biochemistry and Biophysics (IJBB"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu10121950"},{"key":"e_1_2_8_14_1","volume":"11","author":"Moran J. M.","year":"2016","journal-title":"Nat. Prod. Commun."},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.31024\/ajpp.2019.5.s1.1"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2021.119436"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph14111069"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1211\/jpp.61.02.0002"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tifs.2017.07.008"},{"key":"e_1_2_8_20_1","first-page":"2542","author":"Amir Abbas Momtazi-Borojeni","year":"2022","journal-title":"Preclinical toxicity assessment of anionic nanoliposomes. Advanced Industrial and Engineering Polymer Research"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.12980\/APJTB.4.2014C980"},{"key":"e_1_2_8_22_1","first-page":"1","volume-title":"Extraction of oleuropein from olive leaves","author":"G\u00fcm\u00fcsbulut G.","year":"2020"},{"key":"e_1_2_8_23_1","first-page":"1","volume":"4","author":"Yateem H.","year":"2014","journal-title":"Optimum conditions for oleuropein extraction from olive leaves"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-0232(02)00905-4"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2006.07.019"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03246200"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajps.2018.05.011"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9RA05636J"},{"key":"e_1_2_8_29_1","first-page":"1","volume-title":"Anticancer activity of Capsicum Annuum: Capsaicin Loaded Nano-Liposomes Model","author":"Al-Samydai A.","year":"2020"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12272-014-0481-7"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu13113995"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2010.08.020"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10544-015-0028-9"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.9b00109"},{"key":"e_1_2_8_35_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0278216","volume":"17","author":"Al-Nema M.","year":"2022","journal-title":"PLoS One"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0039-9140(97)00375-5"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2006.01.075"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lwt.2021.112093"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.7324\/JAPS.2016.600116"},{"key":"e_1_2_8_40_1","volume-title":"In: Evaluation Nano-Phytosome of Myricetin with Thin Layer Film Hydration-Sonication Method","author":"Saputra Y. E."},{"key":"e_1_2_8_41_1","doi-asserted-by":"crossref","first-page":"17","DOI":"10.22270\/jddt.v7i5.1513","volume":"7","author":"Chivte P. S.","year":"2017","journal-title":"J. Drug Delivery and Therapeutics"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu14235170"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00210-020-02015-9"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10616-009-9191-2"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11094-020-02133-x"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/8867669"}],"container-title":["Chemistry &amp; Biodiversity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.202300534","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,20]],"date-time":"2024-01-20T14:09:47Z","timestamp":1705759787000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbdv.202300534"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8]]},"references-count":46,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2023,8]]}},"alternative-id":["10.1002\/cbdv.202300534"],"URL":"https:\/\/doi.org\/10.1002\/cbdv.202300534","archive":["Portico"],"relation":{},"ISSN":["1612-1872","1612-1880"],"issn-type":[{"value":"1612-1872","type":"print"},{"value":"1612-1880","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,8]]},"assertion":[{"value":"2023-04-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-07-26","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-08-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e202300534"}}