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The ligand\u2010functionalized nanoparticles used in the nano\u2010drug delivery system offer enormous potential for cancer treatments. This work devised a promising approach to increase drug loading efficacy and produce sustained release of 5\u2010fluorouracil (5\u2010FU) and <jats:italic>Ganoderic acid<\/jats:italic> (GA) as model drugs for breast cancer. Chitosan, aptamer, and carbon quantum dot (CS\/Apt\/COQ) hydrogels were initially synthesized as a pH\u2010sensitive and biocompatible delivery system. Then, CS\/Apt\/COQ NPs loaded with 5\u2010FU\u2010GA were made using the W\/O\/W emulsification method. FT\u2010IR, XRD, DLS, zeta potentiometer, and SEM were used to analyze NP's chemical structure, particle size, and shape. Cell viability was measured using MTT assays in\u2005vitro using the MCF\u20107 cell lines. Real\u2010time PCR measured cell apoptotic gene expression. XRD and FT\u2010IR investigations validated nanocarrier production and revealed their crystalline structure and molecular interactions. DLS showed that nanocarriers include NPs with an average size of 250.6\u2005nm and PDI of 0.057. SEM showed their spherical form, and zeta potential studies showed an average surface charge of +37.8\u2005mV. pH\u20055.4 had a highly effective and prolonged drug release profile, releasing virtually all 5\u2010FU and GA in 48\u2005h. Entrapment efficiency percentages for 5\u2010FU and GA were 84.7\u00b15.2 and 80.2\u2009%\u00b12.3, respectively. The 5\u2010FU\u2010GA\u2010CS\u2010CQD\u2010Apt group induced the highest cell death, with just 57.9\u2009% of the MCF\u20107 cells surviving following treatment. 5\u2010FU and GA in CS\u2010CQD\u2010Apt enhanced apoptotic induction by flow cytometry. 5\u2010FU\u2010GA\u2010CS\u2010CQD\u2010Apt also elevated Caspase 9 and downregulated Bcl2. Accordingly, the produced NPs may serve as pH\u2010sensitive nano vehicles for the controlled release of 5\u2010FU and GA in treating breast cancer.<\/jats:p>","DOI":"10.1002\/cbdv.202300659","type":"journal-article","created":{"date-parts":[[2023,8,7]],"date-time":"2023-08-07T13:04:56Z","timestamp":1691413496000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Targeted Anticancer Drug Delivery Using Chitosan, Carbon Quantum Dots, and Aptamers to Deliver Ganoderic Acid and 5\u2010Fluorouracil"],"prefix":"10.1002","volume":"20","author":[{"given":"Aynaz","family":"Mazandarani","sequence":"first","affiliation":[{"name":"Department of Molecular and cell Biology Faculty of Basic Sciences University of Mazandaran  47416-95447 Babolsar Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6391-2195","authenticated-orcid":false,"given":"Ali","family":"Taravati","sequence":"additional","affiliation":[{"name":"Department of Molecular and cell Biology Faculty of Basic Sciences University of Mazandaran  47416-95447 Babolsar Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javad","family":"Mohammadnejad","sequence":"additional","affiliation":[{"name":"Department of Life Science Engineering Faculty of New Sciences and Technology University of Tehran  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatemeh","family":"Yazdian","sequence":"additional","affiliation":[{"name":"Department of Life Science Engineering Faculty of New Sciences and Technology University of Tehran  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,9,5]]},"reference":[{"key":"e_1_2_11_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2020.111781"},{"key":"e_1_2_11_2_1","doi-asserted-by":"crossref","first-page":"1941","DOI":"10.1002\/ijc.31937","volume":"144","author":"Ferlay J.","year":"2018","journal-title":"Int. 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