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The study aims to identify the <jats:italic>in\u2010vitro<\/jats:italic> cytotoxic and acetylcholinesterase activities of <jats:italic>Anthemis tricolor<\/jats:italic>. The compounds responsible for the activities were also identified. Potent extracts of <jats:italic>A. tricolor<\/jats:italic> were subjected to preparative isolation and spectral structure determination studies. The chloroform extract contained many components, and due to the small quantity of extract available, enough pure compound(s) cannot be obtained for structure determination studies, though the <jats:italic>n\u2010<\/jats:italic>hexane extract afforded two known compounds, totarol (<jats:bold>1<\/jats:bold>) and taraxasterol (<jats:bold>2<\/jats:bold>). The structures of the compounds (<jats:bold>1<\/jats:bold> and <jats:bold>2<\/jats:bold>) were determined by <jats:sup>1<\/jats:sup>H and <jats:sup>13<\/jats:sup>C NMR experiments. The pure compounds were also tested for their acetylcholinesterase inhibitory properties. For compound <jats:bold>1<\/jats:bold>, the IC<jats:sub>50<\/jats:sub> value was found to be 87.88\u2005\u03bcg\/mL. However, no inhibition was seen for <jats:bold>2<\/jats:bold>. <jats:italic>Anthemis tricolor<\/jats:italic> was established to be a valuable source of pharmacologically active compounds and requires further studies.<\/jats:p>","DOI":"10.1002\/cbdv.202300913","type":"journal-article","created":{"date-parts":[[2023,12,20]],"date-time":"2023-12-20T12:14:06Z","timestamp":1703074446000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Anthemis tricolor Containing Unusual Totarol with Cytotoxic and Acetylcholinesterase\u2010Inhibitory Activity"],"prefix":"10.1002","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6595-6850","authenticated-orcid":false,"given":"Yasemin","family":"Y\u00fccel Y\u00fccel","sequence":"first","affiliation":[{"name":"Department of Biochemistry Faculty of Pharmacy Altinbas University  Istanbul T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H\u00fcseyin","family":"Servi","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy and Phytotherapy Faculty of Pharmacy Istanbul Yeni Yuzy\u0131l University  Istanbul T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaan","family":"Polato\u011flu","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry Faculty of Pharmacy Altinbas University  Istanbul T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ay\u015fe","family":"Nalbantsoy","sequence":"additional","affiliation":[{"name":"Department of Bioengineering Faculty of Engineering Ege University  Izmir T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,1,9]]},"reference":[{"key":"e_1_2_9_1_1","unstructured":"R.\u2005E. 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