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Synthesizing of thiadiazolo[3,2\u2010a] pyrimidines was carried out in a single step, one\u2010pot reaction using aromatic aldehydes, ethyl acetoacetate and different derivatives of 1,3,4\u2010thiadiazoles (with molar ratio of 1\u2009:\u20092\u2009:\u20091, respectively) in conjunction with the catalyst, anhydrous iron(III) chloride by a grinding method under solvent\u2010free conditions at room temperature. The <jats:italic>in\u2010vitro<\/jats:italic> studies exhibited good potency for inhibiting AChE comparable with donepezil as the reference drug. The best results were obtained by Ethyl 2\u2010(4\u2010nitroophenyl)\u20107\u2010methyl\u20105\u2010(pyridin\u20103\u2010yl)\u20105H\u2010[1,3,4]thiadiazolo[3,2\u2010a]pyrimidine\u20106\u2010carboxylate <jats:bold>4n<\/jats:bold> with IC<jats:sub>50<\/jats:sub> value of 0.082\u00b10.001\u2005\u03bcM which was comparable with AChE inhibitory effects of donepezil (IC<jats:sub>50<\/jats:sub>=0.079\u2005\u03bcM).<\/jats:p>","DOI":"10.1002\/cbdv.202301193","type":"journal-article","created":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T08:48:48Z","timestamp":1698050928000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Biological Evaluation of Anti\u2010Cholinesterase Activity, in Silico Molecular Docking Studies, and DFT Calculations of Green Synthesized Thiadiazolo[3,2\u2010<i>a<\/i>]pyrimidine Derivatives"],"prefix":"10.1002","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0541-8943","authenticated-orcid":false,"given":"Behjat","family":"Pouramiri","sequence":"first","affiliation":[{"name":"Department of Organic Chemistry Qom University of Technology  Qom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohsen","family":"Rashidi","sequence":"additional","affiliation":[{"name":"Department of Chemistry Faculty of Science Shahid Bahonar University of Kerman  Kerman 37195 Qom Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Safa","family":"Lotfi","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Institute of Science and High Technology and Environmental Sciences Graduate University of Advanced Technology  Kerman Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahnaz","family":"Mohammadi","sequence":"additional","affiliation":[{"name":"Department of Physics Qom University of Technology  Qom Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khadijeh","family":"Rabiei","sequence":"additional","affiliation":[{"name":"Department of Organic Chemistry Qom University of Technology  Qom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,11,10]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2014.09.081"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.2174\/156720506779025233"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1022869222652"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2125.1999.00026.x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.med.57.121304.131442"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1745-7254.2007.00664.x"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr040415t"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules28155911"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2017.04.021"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.202300241"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/cryst13050767"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/10406638.2022.2124286"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.201900493"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.202200157"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2004.06.014"},{"key":"e_1_2_8_16_1","first-page":"869","volume":"9","author":"Fellahi Y.","year":"1996","journal-title":"Bull. 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