{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T01:19:45Z","timestamp":1778807985459,"version":"3.51.4"},"reference-count":59,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,11,14]],"date-time":"2023-11-14T00:00:00Z","timestamp":1699920000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Genus <jats:italic>Corydalis<\/jats:italic> is a rich source of isoquinoline alkaloids reported to having potential bioactivities. <jats:italic>Corydalis chaerophylla<\/jats:italic> collected from Nepal at an altitude of 2400\u20134800 m was extracted using hexane, methanol and chloroform as solvents. The resulting hexane, methanol and chloroform extracts were subjected to LC\u2013DAD\u2010MS<jats:sup>n<\/jats:sup> analysis to yield fifteen different alkaloids. To assess any potential pharmacological properties, antimicrobial activity against two Gram\u2010positive, two Gram\u2010negative bacterial strains and one fungal strain was assessed, revealing significant inhibitive action of the methanol and chloroform extracts. Of the extracts obtained using chloroform contained the highest content of phenolic compounds at 113\u2005mg GAE\/g, while the highest total flavonoid content was found for the hexane extract with a value of 46.45\u2005mg QE\/g. The chloroform extract also exhibited a considerable antioxidant activity at IC<jats:sub>50<\/jats:sub> value, 261.5\u00b13\u2005\u03bcg\/mL, for the DPPH assay. Conversely, the methanol extract exhibited the highest LC<jats:sub>50<\/jats:sub> value for Brine Shrimp cytotoxicity at 196\u00b13\u2005\u03bcg\/mL being least potential for the test. The methanol extract was found to be the most active against \u03b1\u2010amylase inhibition with an IC<jats:sub>50<\/jats:sub> of 51.52\u00b12\u2005\u03bcg\/mL. In an <jats:italic>in\u2005vivo<\/jats:italic> acute oral toxicity study against mice, methanol and chloroform extracts presented harmful effects with 1000.36\u2005mg\/kg BW and 515\u2005mg\/kg BW for LD<jats:sub>50<\/jats:sub>, respectively. By analyzing all the results of the solvents used, the chloroform extract was found to be the most active, a feature that will be used in future isolation procedures and other pharmacological tests.<\/jats:p>","DOI":"10.1002\/cbdv.202301209","type":"journal-article","created":{"date-parts":[[2023,11,14]],"date-time":"2023-11-14T14:04:24Z","timestamp":1699970664000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Chemical Characterization of <i>Corydalis chaerophylla<\/i> D.C. 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