{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,10]],"date-time":"2025-09-10T21:42:36Z","timestamp":1757540556543,"version":"3.41.2"},"reference-count":35,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T00:00:00Z","timestamp":1698105600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim of this work is to optimise the antibacterial activity of essential oils (EOs) from <jats:italic>Eucalyptus camaldulensis<\/jats:italic> (ECEO), <jats:italic>Mentha pulegium<\/jats:italic> (MPEO) and <jats:italic>Rosmarinus officinalis<\/jats:italic> (ROEO) plants against <jats:italic>Salmonella<\/jats:italic> spp. and <jats:italic>Escherichia coli<\/jats:italic>. The qualitative antimicrobial effect was assessed using the disc diffusion method, the broth microdilution method was used to determine the minimum inhibitory concentrations (MIC). Polynomial models were created using an augmented centroid simplex mixture design to highlight the synergy of EOs. The results show a significant antibacterial effect of ECEO and MPEO against both bacterial strains, with inhibition zones (IZs) of 13 and 12\u2005mm respectively against <jats:italic>E. coli<\/jats:italic>, and 13 and 11\u2005mm against <jats:italic>Salmonella<\/jats:italic> spp. The latter strain showed a MIC of 0.625\u2009% (v\u2009:\u2009v) by the ECEO, while <jats:italic>E. coli<\/jats:italic> exhibited a MIC of 0.0781\u2009% (v\u2009:\u2009v). The binary combinations of essential oils display a synergistic effect, the proportions of the optimum EOs in the mixture giving the lowest MIC<jats:sub>m<\/jats:sub> were of the order of 50.51\u2009% ECEO and 49.49\u2009% ROEO against <jats:italic>Salmonella<\/jats:italic> spp. and around 50\u2009% MPEO and 50\u2009% ECEO against <jats:italic>E. coli<\/jats:italic>. These results indicate the effectiveness of binary combinations EOs against resistant bacterial strains and suggest their importance in bacterial infections treatment.<\/jats:p>","DOI":"10.1002\/cbdv.202301221","type":"journal-article","created":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T02:54:07Z","timestamp":1696301647000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Optimisation of Three Essential Oils against <i>Salmonella<\/i> spp. and <i>Escherichia coli<\/i> by Mixture Designa"],"prefix":"10.1002","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1755-9882","authenticated-orcid":false,"given":"Rabie","family":"Kachkoul","sequence":"first","affiliation":[{"name":"Laboratory of Biochemistry Faculty of Medicine Pharmacy and Dental Medicine University Sidi Mohammed Ben Abdellah, BP  1893 Km 22, Road of Sidi Harazem Fez Morocco"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ghita","family":"Benjelloun Touimi","sequence":"additional","affiliation":[{"name":"Laboratory of Human Pathology Biomedicine and Environment Faculty of Medicine, Pharmacy and Dental Medicine University Sidi Mohammed Ben Abdellah, BP  1893 Km 22, Road of Sidi Harazem Fez Morocco."},{"name":"Euromed research center, Euromed faculty of medicine Euromed University of Fes (UEMF)  30\u00a0030 Meknes Road, Campus UEMF, BP51 Fez Morocco."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bahia","family":"Bennani","sequence":"additional","affiliation":[{"name":"Laboratory of Human Pathology Biomedicine and Environment Faculty of Medicine, Pharmacy and Dental Medicine University Sidi Mohammed Ben Abdellah, BP  1893 Km 22, Road of Sidi Harazem Fez Morocco."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ghita","family":"El Mouhri","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry Faculty of Medicine Pharmacy and Dental Medicine University Sidi Mohammed Ben Abdellah, BP  1893 Km 22, Road of Sidi Harazem Fez Morocco"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Radouane","family":"El Habbani","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry Faculty of Medicine Pharmacy and Dental Medicine University Sidi Mohammed Ben Abdellah, BP  1893 Km 22, Road of Sidi Harazem Fez Morocco"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aziz","family":"Zouhri","sequence":"additional","affiliation":[{"name":"Bioactives and Environmental Health Laboratory Faculty of Sciences Moulay Ismail University  Meknes B.P. 11201 Morocco."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yahya","family":"El\u2010mernissi","sequence":"additional","affiliation":[{"name":"Research unit in applied chemistry Faculty of Science and Techniques Abdelmalek Essaadi University  Al Hoceima 32003 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