{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T20:20:44Z","timestamp":1787170844684,"version":"build-2736575974"},"reference-count":38,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,2,9]],"date-time":"2024-02-09T00:00:00Z","timestamp":1707436800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The present study shows the untargeted metabolite profiling and\n                    <jats:italic>in\u2005vitro<\/jats:italic>\n                    antibacterial, cytotoxic, and nitric oxide (NO) inhibitory activities of the methanolic leaves extract (MLE) and methanolic stem extract (MSE) of\n                    <jats:italic>Erythroxylum mexicanum<\/jats:italic>\n                    , as well as the fractions from MSE. Using ultra\u2010high performance liquid chromatography\/quadrupole time\u2010of\u2010flight tandem mass spectrometry (UHPLC\u2010QTOF\u2010MS\/MS), a total of 70 metabolites were identified; mainly alkaloids in the MLE, while the MSE showed a high abundance of diterpenoids. The MSE fractions exhibited differential activity against Gram\u2010positive bacteria. Notably, the hexane fraction (HSF) against Streptococcus\n                    <jats:italic>pyogenes<\/jats:italic>\n                    ATCC 19615 (MIC=62.5\u2005\u03bcg\/mL) exhibited a bactericidal effect. The MSE fractions exhibited cytotoxicity against all cancer cell lines tested, with selectivity towards them compared to a noncancerous cell line. Particularly, the HSF and chloroform fraction (CSF) showed the highest cytotoxicity against prostate cancer (PC\u20103) cells, with IC\n                    <jats:sub>50<\/jats:sub>\n                    values of 19.9 and 18.1\u2005\u03bcg\/mL and selectivity indexes of 3.8 and 4.2, respectively. Both the HSF and ethyl acetate (EASF) fractions of the MSE inhibited NO production in RAW 264.7 macrophages, with NO production percentages of 50.0\u2009% and 51.7\u2009%, respectively, at a concentration of 30\u2005\u03bcg\/mL. These results indicated that\n                    <jats:italic>E. mexicanum<\/jats:italic>\n                    can be a source of antibacterial, cytotoxic, and anti\u2010inflammatory metabolites.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202301474","type":"journal-article","created":{"date-parts":[[2024,1,12]],"date-time":"2024-01-12T14:04:54Z","timestamp":1705068294000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Metabolites Profile of Extracts and Fractions of\n                    <i>Erythroxylum mexicanum<\/i>\n                    Kunth by UHPLC\u2010QTOF\u2010MS\/MS and its Antibacterial, Cytotoxic and Nitric Oxide Inhibitory Activities"],"prefix":"10.1002","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2195-2169","authenticated-orcid":false,"given":"Israel","family":"Hurtado\u2010D\u00edaz","sequence":"first","affiliation":[{"name":"Department of Wood Pulp and Paper University Center of Exact Sciences and Engineering University of Guadalajara  Km 15.5 Guadalajara-Nogales, Col. Las Agujas 45200 Zapopan Jalisco Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4696-7359","authenticated-orcid":false,"given":"M. \u00c1ngeles","family":"Ram\u00edrez\u2010Cisneros","sequence":"additional","affiliation":[{"name":"Chemical Research Center-IICBA Autonomous University of the State of Morelos  Av. Universidad 1001, Chamilpa 62209 Cuernavaca Morelos Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7464-9898","authenticated-orcid":false,"given":"Laura","family":"Alvarez","sequence":"additional","affiliation":[{"name":"Chemical Research Center-IICBA Autonomous University of the State of Morelos  Av. Universidad 1001, Chamilpa 62209 Cuernavaca Morelos Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6555-6267","authenticated-orcid":false,"given":"Jessica Nayelli","family":"S\u00e1nchez\u2010Carranza","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy Autonomous University of the State of Morelos  Av. Universidad 1001, Chamilpa 62209 Cuernavaca Morelos M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4600-4299","authenticated-orcid":false,"given":"Mar\u00eda Crystal","family":"Columba\u2010Palomares","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy Autonomous University of the State of Morelos  Av. Universidad 1001, Chamilpa 62209 Cuernavaca Morelos M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Antonio","family":"Silva\u2010Guzm\u00e1n","sequence":"additional","affiliation":[{"name":"Department of Wood Pulp and Paper University Center of Exact Sciences and Engineering University of Guadalajara  Km 15.5 Guadalajara-Nogales, Col. Las Agujas 45200 Zapopan Jalisco Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7127-7264","authenticated-orcid":false,"given":"Francisco","family":"Cruz\u2010Sosa","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Autonomous Metropolitan University-Iztapalapa Campus  Av. Ferrocarril de San Rafael Atlixco 186, Col. Leyes de Reforma 1a. Secci\u00f3n, Alcald\u00eda Iztapalapa Mexico City 09310 Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2811-1143","authenticated-orcid":false,"given":"Antonio","family":"Bernab\u00e9\u2010Antonio","sequence":"additional","affiliation":[{"name":"Department of Wood Pulp and Paper University Center of Exact Sciences and Engineering University of Guadalajara  Km 15.5 Guadalajara-Nogales, Col. 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