{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T03:20:23Z","timestamp":1791688823551,"version":"4.3.4"},"reference-count":40,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,11,28]],"date-time":"2023-11-28T00:00:00Z","timestamp":1701129600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Cyclophosphamide (CYP) is commonly used to treat cancer of the ovaries, breast, lymph, and blood system and produces interstitial cystitis (IC) via its urotoxic metabolite: i.\u2009e., acrolein. The present study was aimed to investigate the uroprotective effect of campesterol (a steroidal phytochemical) in cyclophosphamide induced IC. IC was induced by CYP (150\u2005mg\/kg,\n                    <jats:italic>i.\u2009p<\/jats:italic>\n                    .) in rats. The Enzyme linked immunosorbent assays for oxidative stress markers and Polymerase Chain Reaction (PCR) for inflammatory cytokines were carried out. The Tissue Organ Bath Technique was used for the evaluation of the spasmolytic effect of campesterol. Different pharmacological antagonists have been used to explore the mechanism of action of campesterol. Treatment with campesterol (70\u2005mg\/kg) reduced nociception (55\u2009%), edema (67\u2009%), hemorrhage (67\u2009%), and protein leakage significantly (94\u2009%). The antioxidant activity of campesterol was exhibited by a fall in MDA, NO, and an elevation in SOD, CAT, and GPX levels. Campesterol presented anti\u2010inflammatory potential by decreasing IL\u20101, TNF\u2010\u03b1, and TGF\u2010\u03b2 expression levels. Histologically, it preserved urothelium from the deleterious effect of CYP. Campesterol showed a spasmolytic effect by reducing bladder overactivity that was dependent on muscarinic receptors, voltage\u2010gated calcium and K\n                    <jats:sub>ATP<\/jats:sub>\n                    channels, and cyclo\u2010oxygenase pathways.\n                    <jats:italic>In silico<\/jats:italic>\n                    studies confirmed the biochemical findings. The findings suggest that campesterol could be valorized as a possible therapeutic agent against cyclophosphamide\u2010induced interstitial cystitis.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202301534","type":"journal-article","created":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T07:07:16Z","timestamp":1700550436000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Uroprotective Potential of Campesterol in Cyclophosphamide Induced Interstitial Cystitis; Molecular Docking Studies"],"prefix":"10.1002","volume":"20","author":[{"given":"Joham","family":"Javed","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy The University of Lahore  Lahore 55150 Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irfan","family":"Anjum","sequence":"additional","affiliation":[{"name":"Department of Basic Medical Sciences, Shifa College of Pharmaceutical Sciences Shifa Tameer-e-Millat University  Islamabad 44000 Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saima","family":"Najm","sequence":"additional","affiliation":[{"name":"Lahore College of Pharmaceutical Sciences Department of Pharmacy  Lahore 55150 Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naila","family":"Ali","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology and Biotechnology The University of Lahore  Lahore 55150 Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Nasir Hayat Malik","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy Capital University of Science and Technology  Islamabad 44000 Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shah","family":"Jahan","sequence":"additional","affiliation":[{"name":"Department of Immunology University of Health Sciences  Lahore 55150 Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Turki M.","family":"Dawoud","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology, College of Science King Saud University  P. O. BOX 2455 Riyadh 11451 Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiba\u2010Allah","family":"Nafidi","sequence":"additional","affiliation":[{"name":"Department of Food Science, Faculty of Agricultural and Food Sciences Laval University, 2325  Quebec City QC G1\u2009V0\u2009A6 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3707-8461","authenticated-orcid":false,"given":"Mohammed","family":"Bourhia","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, Faculty of Medicine and Pharmacy Ibn Zohr University  Laayoune 70000 Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,11,28]]},"reference":[{"key":"e_1_2_10_1_1","first-page":"34033350.2021","volume-title":"Interstitial Cystitis. 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