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Arthritis was induced by complete Freund's adjuvant (CFA) and its severity was assessed by the arthritis index. Treatment with MS seeds butanol fraction and interlukin\u20101 receptor antagonist (IL\u20101RA) were evaluated through measuring interlukin\u20101 receptor (IL\u20101R) type 1 gene expression, interlukin\u20101 beta (IL\u20101\u03b2), oxidative stress markers, C\u2010reactive protein (CRP), tumor necrosis factor\u2010alpha (TNF\u2010\u03b1), prostaglandin E2 (PGE2), caspase\u20103 (Cas\u20103), intracellular adhesion molecule\u20101 (ICAM\u20101), DNA fragmentation, and chromosomal damage. Total phenolics\/ flavonoids content in the ethyl acetate, butanol fraction and crude extract of MS seeds were estimated. The major identified compounds were Quercetin, <jats:italic>Trans<\/jats:italic>\u2010taxifolin, Gallic acid, 7,4\u2032\u2010Dihydroxyflavone, Cinnamic acid, Kudzusaponin SA4, Isorhamnetin 3\u2010O\u2010beta\u2010D\u20102\u2032\u2032,3\u2032\u2032,4\u2032\u2032\u2010triacetylglucopyranoside, Apigenin, 5,7,4\u2032\u2010Trihydroxy\u20103\u2032\u2010methoxyflavone, Desmethylxanthohumol, Pantothenic acid, Soyasapogenol E, Malvidin, Helilandin B, Stigmasterol, and Wairol. Treatment with MS seeds butanol fraction and IL\u20101RA enhanced all the biochemical parameters and the histopathological features of the ankle joint. In conclusion, <jats:italic>Trans<\/jats:italic>\u2010taxifolin was isolated for the first time from the genus Medicago. MS butanol fraction seeds extract and IL\u20101 RA were considered as anti\u2010rheumatic agents.<\/jats:p>","DOI":"10.1002\/cbdv.202301653","type":"journal-article","created":{"date-parts":[[2023,12,30]],"date-time":"2023-12-30T06:54:58Z","timestamp":1703919298000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Inflammatory mediators, oxidative stress and genetic disturbance in rheumatoid arthritis rats supported by alfalfa seeds metabolomic constituents <i>via<\/i> blocking interleukin\u20101receptor."],"prefix":"10.1002","volume":"21","author":[{"given":"Asmaa F.","family":"Aboul Naser","sequence":"first","affiliation":[{"name":"Department of Therapeutic Chemistry National Research Centre  Dokki Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yomna R.","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Department of Therapeutic Chemistry National Research Centre  Dokki Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mona A.","family":"Mohammed","sequence":"additional","affiliation":[{"name":"Medicinal and Aromatic Plants Research Department National Research Centre  Dokki Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed","family":"Aboelmagd","sequence":"additional","affiliation":[{"name":"Pharmacognosy Department National Research Centre  Dokki Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mona E.","family":"Aboutabl","sequence":"additional","affiliation":[{"name":"Medicinal and Pharmaceutical Chemistry Department (Pharmacology Group) National Research Centre  Dokki Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Entesar E.","family":"Hassan","sequence":"additional","affiliation":[{"name":"Department of Genetics and Cytology National Research Centre  Dokki Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wagdy K. 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