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In this study, we used rosmarinic acid (RosA) to prepare Cu<jats:sub>0.5<\/jats:sub>Zn<jats:sub>0.5<\/jats:sub>FeO<jats:sub>4<\/jats:sub> NPs and later encapsulated them using PEG polymer. Characterization of NPs was done using the XRD method and SEM imaging. Further, we explored the encapsulated NPs for anti\u2010inflammatory properties by downregulating the expression of pro\u2010inflammatory cytokines mRNA in LPS\u2010stimulated Raw 264.7 cells. Besides, employing DPPH, NO and ABTS radical scavenging assays to examine the antioxidant activity of the synthesized Cu<jats:sub>0.5<\/jats:sub>Zn<jats:sub>0.5<\/jats:sub>FeO<jats:sub>4<\/jats:sub> NPs. Cu0.5Zn0.5FeO4 NPs revealed moderate antioxidant activity by scavenging DPPH and nitric oxide. We demonstrated that the NPs showed high potential anti\u2010inflammatory activity by suppressing the mRNA and protein levels of pro\u2010inflammatory cytokines in a dose\u2010dependent manner, in LPS\u2010induced Raw 264.7 cells. To our best knowledge, this is the first report where RosA was found to be a suitable phyto source for the green synthesis of Cu0.5Zn0.5FeO4 NPs and their <jats:italic>in\u2005vitro<\/jats:italic> anti\u2010inflammatory and antioxidant effects. Taken together, our findings suggest that the RosA is a green resource for the eco\u2010friendly synthesis of Cu0.5Zn0.5FeO4\/PEG NPs, which further can be employed as a novel anti\u2010inflammatory therapeutic agent.<\/jats:p>","DOI":"10.1002\/cbdv.202301739","type":"journal-article","created":{"date-parts":[[2024,1,20]],"date-time":"2024-01-20T07:48:01Z","timestamp":1705736881000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Anti\u2010Inflammatory and Antioxidant Effects of Rosmarinic Acid Trimetallic (Cu<sub>0.5<\/sub>Zn<sub>0.5<\/sub>Fe<sub>2<\/sub>O<sub>4<\/sub>) Nanoparticles"],"prefix":"10.1002","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1191-2905","authenticated-orcid":false,"given":"Amer","family":"Imraish","sequence":"first","affiliation":[{"name":"Department of Biological Sciences School of Science The University of Jordan  Queen Rania Al-Abdullah Street Amman 11942 Jordan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Malek","family":"Zihlif","sequence":"additional","affiliation":[{"name":"Department of Pharmacology School of Medicine The University of Jordan  Queen Rania Al-Abdullah Street Amman 11942 Jordan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tuqa","family":"Abu Thiab","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences School of Science The University of Jordan  Queen Rania Al-Abdullah Street Amman 11942 Jordan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wajdy","family":"Al\u2010Awaida","sequence":"additional","affiliation":[{"name":"Department of Biology and Biotechnology Faculty of Science American University of Madaba  P.O. 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