{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T22:14:58Z","timestamp":1791497698749,"version":"4.3.4"},"reference-count":105,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,1,22]],"date-time":"2024-01-22T00:00:00Z","timestamp":1705881600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The present study focused on the synthesis and characterization of novel pyrazole carboxamide derivatives (\n                    <jats:bold>SA1\u201012<\/jats:bold>\n                    ). The inhibitory effect of the compounds on cholinesterases (ChEs; AChE and BChE) and carbonic anhydrases (\n                    <jats:italic>h<\/jats:italic>\n                    CAs;\n                    <jats:italic>h<\/jats:italic>\n                    CA I and\n                    <jats:italic>h<\/jats:italic>\n                    CA II) isoenzymes were screened as\n                    <jats:italic>in\u2005vitro<\/jats:italic>\n                    . These series compounds have been identified as potential inhibitors with a\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>I<\/jats:sub>\n                    values in the range of 10.69\u00b11.27\u201370.87\u00b18.11\u2005nM for hCA I, 20.01\u00b13.48\u201356.63\u00b16.41\u2005nM for hCA II, 6.60\u00b10.62\u201014.15\u00b11.09\u2005nM for acetylcholinesterase (AChE) and 54.87\u00b17.76\u2013137.20 \u00b19.61\u2005nM for butyrylcholinesterase (BChE). These compounds have a more effective inhibition effect when compared to the reference compounds. In addition, the potential binding positions of the compounds with high affinity for ChE and hCAs were demonstrated by\n                    <jats:italic>in silico<\/jats:italic>\n                    methods. The results of\n                    <jats:italic>in silico<\/jats:italic>\n                    and\n                    <jats:italic>in vitro<\/jats:italic>\n                    studies support each other. As a result of the present study, the compounds with high inhibitory activity for metabolic enzymes, such as ChE and hCA were designed. The compounds may be potential alternative agents used as selective ChE and hCA inhibitors in the treatment of Alzheimer\u2032s disease and glaucoma.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202301824","type":"journal-article","created":{"date-parts":[[2023,12,27]],"date-time":"2023-12-27T06:28:52Z","timestamp":1703658532000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Design and Synthesis of Pyrazole Carboxamide Derivatives as Selective Cholinesterase and Carbonic Anhydrase Inhibitors: Molecular Docking and Biological Evaluation"],"prefix":"10.1002","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3012-7582","authenticated-orcid":false,"given":"Mustafa","family":"Durgun","sequence":"first","affiliation":[{"name":"Department of Chemistry Faculty of Arts and Sciences Harran University  63290 \u015eanl\u0131urfa Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suleyman","family":"Akocak","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry Faculty of Pharmacy Ad\u0131yaman University  02040 Ad\u0131yaman Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nebih","family":"Lolak","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry Faculty of Pharmacy Ad\u0131yaman University  02040 Ad\u0131yaman Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fevzi","family":"Topal","sequence":"additional","affiliation":[{"name":"Department of Food Engineering Faculty of Engineering and Natural Sciences G\u00fcm\u00fc\u015fhane University  29100 G\u00fcm\u00fc\u015fhane Turkey"},{"name":"Department of Chemical and Chemical Processing Technologies G\u00fcm\u00fc\u015fhane Vocational School G\u00fcm\u00fc\u015fhane University  29100 G\u00fcm\u00fc\u015fhane Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00dcmit Muhammet","family":"Ko\u00e7yi\u011fit","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Faculty of Pharmacy Sivas Cumhuriyet University  58140 Sivas Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00fcneyt","family":"T\u00fcrke\u015f","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Faculty of Pharmacy Erzincan Binali Y\u0131ld\u0131r\u0131m University  24002 Erzincan Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mesut","family":"I\u015f\u0131k","sequence":"additional","affiliation":[{"name":"Department of Bioengineering Faculty of Engineering Bilecik \u015eeyh Edebali University  11230 Bilecik Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u015e\u00fckr\u00fc","family":"Beydemir","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Faculty of Pharmacy Anadolu University  26470 Eski\u015fehir Turkey"},{"name":"Bilecik \u015eeyh Edebali University  11230 Bilecik Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,1,22]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd2467"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.etap.2011.03.013"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1042\/CS20210040"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2015.02.027"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr050262p"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/13543776.2018.1523897"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2020.104204"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.0c01390"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.4155\/fmc-2021-0222"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2007.04.020"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1517\/13543776.2012.707646"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo8010020"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1350-9462(99)00006-3"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/14756366.2016.1187605"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.117.5.573"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctrv.2012.08.004"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.8b00208"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2022.105920"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1042\/BCJ20160115"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr200176r"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/med.21497"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/ardp.201900383"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/npp.2016.81"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.12.085"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.14476"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/316345a0"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1092852900014164"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.3109\/14756366.2015.1135914"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12031-013-0176-4"},{"key":"e_1_2_9_30_1","volume":"240","author":"Zhang H.","year":"2022","journal-title":"Eur. J. Med. Chem."},{"key":"e_1_2_9_31_1","first-page":"1908","volume-title":"Hayes\u2032 handbook of pesticide toxicology","author":"Krieger R.","year":"2010"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-19701-7"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2021.105149"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.13975"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1080\/13813455.2019.1683584"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2020.109287"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0742-8413(00)00150-X"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2013.09.050"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom10010040"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clineuro.2020.105959"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11064-019-02852-y"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2019.05.109"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.31887\/DCNS.2000.2.2\/lschneider"},{"key":"e_1_2_9_44_1","volume-title":"Heterocyclic Chemistry: Volume II: Five-Membered Heterocycles","author":"Gupta R. R.","year":"2013"},{"key":"e_1_2_9_45_1","first-page":"331","volume-title":"Knorr pyrazole synthesis, In Name Reactions: A Collection of Detailed Reaction Mechanisms","author":"Li J. J.","year":"2006"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6NJ03181A"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr2000459"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2022.105969"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2021.113351"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200006293422603"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1039\/D2MD00206J"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.2600730503"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-4733.2007.00281.x"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/0028-3908(78)90108-9"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0277-5387(96)00346-4"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/0026-0495(82)90114-7"},{"key":"e_1_2_9_57_1","first-page":"22","volume-title":"Chapter\u20053 Antidepressant Agents. In Annu. Rep. Med. Chem.","author":"Luttinger D.","year":"1987"},{"key":"e_1_2_9_58_1","first-page":"2869","volume":"40","author":"Olah E.","year":"1980","journal-title":"Cancer Res."},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-3542(82)90002-X"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2020.103647"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1002\/ardp.201800200"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1080\/07391102.2021.1916599"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11030-022-10527-0"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tetlet.2014.01.052"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2009.10.035"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2019.103213"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jscs.2016.05.008"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2019.02.013"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1080\/14756366.2019.1695791"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1080\/14756366.2022.2103553"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molstruc.2021.131591"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2020.112273"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1080\/14756366.2018.1488696"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm00158a028"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/162928"},{"key":"e_1_2_9_76_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2022.105822"},{"key":"e_1_2_9_77_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2022.05.009"},{"key":"e_1_2_9_78_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20130013"},{"key":"e_1_2_9_79_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1994.1655"},{"key":"e_1_2_9_80_1","doi-asserted-by":"publisher","DOI":"10.1107\/S1744309109036665"},{"key":"e_1_2_9_81_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(61)90145-9"},{"key":"e_1_2_9_82_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2021.116320"},{"key":"e_1_2_9_83_1","doi-asserted-by":"publisher","DOI":"10.1080\/07391102.2020.1857842"},{"key":"e_1_2_9_84_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac9002722"},{"key":"e_1_2_9_85_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)95800-X"},{"key":"e_1_2_9_86_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)99681-X"},{"key":"e_1_2_9_87_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2020.103897"},{"key":"e_1_2_9_88_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi201284u"},{"key":"e_1_2_9_89_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)39978-7"},{"key":"e_1_2_9_90_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja01318a036"},{"key":"e_1_2_9_91_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jchemed.9b00396"},{"key":"e_1_2_9_92_1","unstructured":"Schr\u00f6dinger Release 2023\u20132: Protein Preparation Wizard Schr\u00f6dinger LLC New York NY 2023."},{"key":"e_1_2_9_93_1","unstructured":"Schr\u00f6dinger Release 2023\u20132: LigPrep Schr\u00f6dinger LLC New York NY 2023."},{"key":"e_1_2_9_94_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jctc.1c00302"},{"key":"e_1_2_9_95_1","unstructured":"Schr\u00f6dinger Release 2023\u20132: Epik Schr\u00f6dinger LLC New York NY 2023."},{"key":"e_1_2_9_96_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10822-007-9133-z"},{"key":"e_1_2_9_97_1","unstructured":"Schr\u00f6dinger Release 2023\u20132: Receptor Grid Generation Schr\u00f6dinger LLC New York NY 2023."},{"key":"e_1_2_9_98_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2021.105473"},{"key":"e_1_2_9_99_1","doi-asserted-by":"publisher","DOI":"10.1080\/07391102.2023.2240889"},{"key":"e_1_2_9_100_1","unstructured":"Schr\u00f6dinger Release 2023\u20132: SiteMap Schr\u00f6dinger LLC New York NY 2023."},{"key":"e_1_2_9_101_1","unstructured":"Schr\u00f6dinger Release 2023\u20132: Glide Schr\u00f6dinger LLC New York NY 2023."},{"key":"e_1_2_9_102_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2023.124232"},{"key":"e_1_2_9_103_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm0306430"},{"key":"e_1_2_9_104_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm030644s"},{"key":"e_1_2_9_105_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep42717"}],"container-title":["Chemistry &amp; Biodiversity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.202301824","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T19:30:50Z","timestamp":1741721450000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbdv.202301824"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,22]]},"references-count":105,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024,2]]}},"alternative-id":["10.1002\/cbdv.202301824"],"URL":"https:\/\/doi.org\/10.1002\/cbdv.202301824","archive":["Portico"],"relation":{},"ISSN":["1612-1872","1612-1880"],"issn-type":[{"value":"1612-1872","type":"print"},{"value":"1612-1880","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,22]]},"assertion":[{"value":"2023-11-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e202301824"}}