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The primary objective of this study was to conduct a phytochemical analysis and assess the multifunctional capabilities of aforementioned plant in dual therapy for Alzheimer\u2032s disease (AD) and Type\u20052 diabetes (T2D). The phytochemical screening of ethanol, methanol extract, and their derived fractions of <jats:italic>Limeum indicum<\/jats:italic> was conducted using GC\u2010MS, HPLC, UV\u2010analysis and FTIR. The antioxidant capacity was evaluated by DPPH method. The inhibitory potential of the extracts\/fractions against \u03b1\u2010, \u03b2\u2010glucosidase acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and monoaminine oxidases (MAO\u2010A &amp; B) was evaluated. Results revealed that acetonitrile fraction has highest inhibitory potential against \u03b1\u2010glucosidase (IC<jats:sub>50<\/jats:sub>=68.47\u00b10.05\u2005\u03bcg\/mL), methanol extract against \u03b2\u2010glucosidase (IC<jats:sub>50<\/jats:sub>=91.12\u00b10.07\u2005\u03bcg\/mL), ethyl acetate fraction against AChE (IC<jats:sub>50<\/jats:sub>=59.0\u00b10.02\u2005\u03bcg\/mL), ethanol extract against BChE (28.41\u00b10.01\u2005\u03bcg\/mL), n\u2010hexane fraction against MAO\u2010A (IC<jats:sub>50<\/jats:sub>=150.5\u00b10.31\u2005\u03bcg\/mL) and methanol extract for MAO\u2010B (IC<jats:sub>50<\/jats:sub>=75.95\u00b10.13\u2005\u03bcg\/mL). The docking analysis of extracts\\fractions suggested the best binding scores within the active pocket of the respective enzymes. During the <jats:italic>in\u2010vivo<\/jats:italic> investigation, ethanol extract produced hypoglycemic effect (134.52\u00b12.79 and 119.38\u00b11.40\u2005mg\/dl) after 21\u2005days treatment at dose level of 250 and 500\u2005mg\/Kg. Histopathological findings further supported the <jats:italic>in\u2010vivo<\/jats:italic> studies.<\/jats:p>","DOI":"10.1002\/cbdv.202301858","type":"journal-article","created":{"date-parts":[[2024,4,12]],"date-time":"2024-04-12T19:33:54Z","timestamp":1712950434000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Investigations of <i>Limeum Indicum<\/i> Plant for Diabetes Mellitus and Alzheimer's Disease Dual Therapy: Phytochemical, GC\u2010MS Chemical Profiling, Enzyme Inhibition, Molecular Docking and In\u2010Vivo Studies"],"prefix":"10.1002","volume":"21","author":[{"given":"Tahira","family":"Shamim","sequence":"first","affiliation":[{"name":"University College of Conventional Medicine Faculty of Medicine &amp; Allied Health Sciences The Islamia University of Bahawalpur  63100 Bahawalpur Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hafiz Muhammad","family":"Asif","sequence":"additional","affiliation":[{"name":"University College of Conventional Medicine Faculty of Medicine &amp; Allied Health Sciences The Islamia University of Bahawalpur  63100 Bahawalpur Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8516-7234","authenticated-orcid":false,"given":"Syeda","family":"Abida Ejaz","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry Faculty of Pharmacy The Islamia University of Bahawalpur  63100 Bahawalpur Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zahid","family":"Hussain","sequence":"additional","affiliation":[{"name":"Department of Chemistry COMSATS University Islamabad  Abbottabad Campus 22060 Abbottabad Pakistan"},{"name":"Center for Advance Drug Research COMSATS University Islamabad  Abbottabad Campus 22060 Abbottabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanveer A.","family":"Wani","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry College of Pharmacy King Saud University  P.O.Box 2452 11451 Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laila","family":"Sumreen","sequence":"additional","affiliation":[{"name":"University College of Conventional Medicine Faculty of Medicine &amp; Allied Health Sciences The Islamia University of Bahawalpur  63100 Bahawalpur Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Abdullah","sequence":"additional","affiliation":[{"name":"Cholistan Institute of Desert Studies The Islamia University of Bahawalpur  63100 Bahawalpur Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zubair","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Department of Pharmacy COMSATS University Islamabad  Abbottabad Campus 22060 Abbottabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jamshed","family":"Iqbal","sequence":"additional","affiliation":[{"name":"Department of Chemistry COMSATS University Islamabad  Abbottabad Campus 22060 Abbottabad Pakistan"},{"name":"Center for Advance Drug Research COMSATS University Islamabad  Abbottabad Campus 22060 Abbottabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Song Ja","family":"Kim","sequence":"additional","affiliation":[{"name":"College of Natural Sciences Department of Biological Sciences Kongju National University  32588 Gongju South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Kamal","family":"Shah","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary and Animal Sciences Gomal University  29220 Dera Ismail Khan Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,5,15]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1080\/07853890.2021.2009124"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2019.04.015"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12264-021-00701-z"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/trc2.12009"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/1061186X.2019.1584808"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389200218666170203110135"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtemb.2021.126779"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.00456"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/cns.12971"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/07391102.2020.1784796"},{"key":"e_1_2_9_11_1","volume":"13","author":"Chen X. 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