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L. were found to have the highest chymotrypsin inhibitory activities (87.75 and 84.24\u2009% inhibition). Similarly, the highest trypsin inhibitory activity was observed in <jats:italic>D.\u2005pontica<\/jats:italic> (%99.93 inhibition), followed by <jats:italic>Sambucus ebulus<\/jats:italic> L. flowers (87.47\u2009% inhibition). Extracts showing strong enzyme inhibition were fractioned and subjected to activity tests. The highest chymotrypsin inhibitory activity was observed in the <jats:italic>n<\/jats:italic>\u2010hexane fraction of <jats:italic>D.\u2005pontica<\/jats:italic> (%80.70 inhibition), while the highest trypsin inhibitory activity was found in the <jats:italic>n<\/jats:italic>\u2010butanol fraction of <jats:italic>S.\u2005ebulus<\/jats:italic> (%86.81 inhibition). HPLC studies determined that the 80\u2009% ethanol extract of <jats:italic>D.\u2005pontica<\/jats:italic> and its dichloromethane and ethyl acetate fractions contained umbelliferone. It was found that chlorogenic acid was present in the 80\u2009% ethanol extracts of <jats:italic>S.\u2005ebulus<\/jats:italic> flowers. <jats:italic>M.\u2005longifolia<\/jats:italic> was found to contain chlorogenic acid, caffeic acid, luteolin\u20107\u2010glucoside, and rosmarinic acid. <jats:italic>M.\u2005longifolia<\/jats:italic> has been identified as the plant exhibiting the highest antioxidant activity in ABTS and CUPRAC tests, consistent with its high phenolic and flavonoid content.<\/jats:p>","DOI":"10.1002\/cbdv.202301879","type":"journal-article","created":{"date-parts":[[2024,1,30]],"date-time":"2024-01-30T12:29:50Z","timestamp":1706617790000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Chymotrypsin and Trypsin Inhibitory Activity of Some Medicinal Plants Collected from Rize (T\u00fcrkiye)"],"prefix":"10.1002","volume":"21","author":[{"given":"Tugba","family":"Gunbatan","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy Faculty of Pharmacy Gazi University  06330 Ankara T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melike","family":"Sucu","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy Faculty of Pharmacy Baskent University  06790 Ankara T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alper","family":"Gokbulut","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy Faculty of Pharmacy 06560 Ankara University  Ankara T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elif","family":"Dilmac","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy Faculty of Pharmacy Gazi University  06330 Ankara T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilhan","family":"Gurbuz","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy Faculty of Pharmacy Gazi University  06330 Ankara T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,2,16]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2020.02.016"},{"key":"e_1_2_8_2_1","unstructured":"\u00a0"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2014.03.007"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0088894"},{"key":"e_1_2_8_5_1","unstructured":"\u00a0"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2016.00261"},{"key":"e_1_2_8_7_2","author":"Krotova K.","year":"2018","journal-title":"bioRxiv"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph13120421"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jnatprod.7b00551"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods8060200"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-006-9589-z"},{"key":"e_1_2_8_12_1","unstructured":"\u00a0"},{"key":"e_1_2_8_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(70)19006-9"},{"key":"e_1_2_8_14_2","first-page":"249","volume-title":"The Enzymes, Vol. III","author":"Keil B.","year":"1971"},{"key":"e_1_2_8_15_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00886a039"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/14756366.2018.1542387"},{"key":"e_1_2_8_17_1","unstructured":"\u00a0"},{"key":"e_1_2_8_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10930-011-9325-1"},{"key":"e_1_2_8_19_2","doi-asserted-by":"publisher","DOI":"10.3390\/healthcare9081002"},{"key":"e_1_2_8_20_1","first-page":"185","volume-title":"The Enzymes, Vol. 3","author":"Blow D. 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