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It contains pharmacologically active compounds that may have neuroprotective, antioxidant, fertility\u2010enhancing, and antiviral effects. Unlike other bee products, the chemical composition of bee drone larva is poorly studied. This study analyzed the chemical compostion of apilarnil using several methods. These included liquid chromatography\u2010mass spectrometry (LC\u2010MS\/MS) and a combination of gas chromatography\/mass spectrometry with solid phase micro\u2010extraction (SPME\/GC\u2010MS). Additionally, antioxidant activity of the apilarnil was assessed using 2,2\u2010diphenyl\u20101\u2010picrylhydrazyl (DPPH) assay. A chemical assessment of apilarnil showed that it has 6.3\u00b10.00, 74.67\u00b10.10\u2009%, 3.65\u00b10.32\u2009%, 8.80\u00b11.01\u2009%, 13.16\u00b10.94\u2009%, and 8.79\u00b10.49\u2009% of pH, moisture, total lipids, proteins, flavonoids, and carbohydrates, respectively. LC\u2010MS\/MS analysis and molecular networking (GNPS) of apilarnil exhibited 44 compounds, including fatty acids, flavonoids, glycerophospholipids, alcohols, sugars, amino acids, and steroids. GC\u2010MS detected 30 volatile compounds in apilarnil, mainly esters (24\u2009%), ketones (23.84\u2009%), ethers (15.05\u2009%), alcohols (11.41\u2009%), fatty acids (10.06), aldehydes (6.73\u2009%), amines (5.46), and alkene (5.53\u2009%). The antioxidant activity of apilarnil was measured using DPPH with an IC<jats:sub>50<\/jats:sub> of 179.93\u00b12.46\u2005\u03bcg\/ml.<\/jats:p>","DOI":"10.1002\/cbdv.202400085","type":"journal-article","created":{"date-parts":[[2024,2,8]],"date-time":"2024-02-08T12:04:18Z","timestamp":1707393858000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Exploring the Chemical Constituents and Nutritive Potential of Bee Drone (Apilarnil): Emphasis on Antioxidant Properties"],"prefix":"10.1002","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2200-3507","authenticated-orcid":false,"given":"Aida A.","family":"Abd El\u2010Wahed","sequence":"first","affiliation":[{"name":"Department of Bee Research Plant Protection Research Institute Agricultural Research Centre  Giza 12627 Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaden A. 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