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In this study, it was aimed to investigate the skin\u2010related biological activities and phytochemical characterization of OA. Moreover, an emulgel formulation was developed from the main methanolic extract of the plant (OAM). Initially, to identifiy of the active fractions, aerial parts of the plant material was extracted with methanol and fractionated by n\u2010hexane, chloroform, ethyl acetate and n\u2010butanol, respectively. Antioxidant activity was determined by CUPRAC, TOAC, FRAP and DPPH assays. Thereafter, the inhibition potential of OAM, novel formulation and all fractions was measured against elastase, collagenase, tyrosinase and hyaluronidase enzymes. OAM was analyzed and characterized by LC\/MS\u2010MS. The major bioactive flavonoids which are rutin and isoquercetin were measured and compared as qualitative and quantitative via high performance thin layer chromatography (HPTLC) analysis in OAM and fractions. The results showed that extracts of OA can be a potential cosmeceutical agent for skin related problems.<\/jats:p>","DOI":"10.1002\/cbdv.202400139","type":"journal-article","created":{"date-parts":[[2024,3,18]],"date-time":"2024-03-18T06:55:10Z","timestamp":1710744910000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<i>In vitro<\/i> Evaluation of Skin Related Enzyme Inhibitory Effect and Emulgel Formulation Development Studies of <i>Onobrychis Argyrea<\/i> subsp. <i>Argyrea<\/i> with Phytochemical Analysis"],"prefix":"10.1002","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0138-2480","authenticated-orcid":false,"given":"Tu\u011fba Buse","family":"\u015eent\u00fcrk","sequence":"first","affiliation":[{"name":"Ac\u0131badem Mehmet Ali Ayd\u0131nlar University Faculty of Pharmacy Department of Pharmacognosy  34752 Ata\u015fehir \u0130stanbul T\u00fcrkiye"},{"name":"University of Health Sciences Hamidiye Faculty of Pharmacy Department of Pharmacognosy  34688 \u0130stanbul T\u00fcrkiye."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7434-3175","authenticated-orcid":false,"given":"Timur Hakan","family":"Barak","sequence":"additional","affiliation":[{"name":"Ac\u0131badem Mehmet Ali Ayd\u0131nlar University Faculty of Pharmacy Department of Pharmacognosy  34752 Ata\u015fehir \u0130stanbul T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2010-4918","authenticated-orcid":false,"given":"Emre \u015eefik","family":"\u00c7a\u011flar","sequence":"additional","affiliation":[{"name":"University of Health Sciences Hamidiye Faculty of Pharmacy Department of Pharmaceutical Biotechnology  34668 \u0130stanbul T\u00fcrkiye."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0250-9084","authenticated-orcid":false,"given":"Ebru \u00d6zdemir","family":"Nath","sequence":"additional","affiliation":[{"name":"Alt\u0131nba\u015f University Natural Products R&amp;D Center  34144 \u0130stanbul T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8362-3136","authenticated-orcid":false,"given":"Zafer \u00d6mer","family":"\u00d6zdemir","sequence":"additional","affiliation":[{"name":"University of Health Sciences Hamidiye Faculty of Pharmacy Department of Analytical Chemistry  34688 \u0130stanbul T\u00fcrkiye."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,4,10]]},"reference":[{"key":"e_1_2_9_1_1","first-page":"359","volume":"25","author":"Aktoklu E.","year":"2001","journal-title":"Turk. 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