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The study evaluated the antifungal activity of 4\u2010chlorobenzyl\n                    <jats:italic>p<\/jats:italic>\n                    \u2010coumarate, an unprecedented semi\u2010synthetic molecule. Docking molecular and assay experiments were conducted to determine the Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC), mode of action, effect on growth, fungal death kinetics, drug association, effects on biofilm, micromorphology, and against human keratinocytes. The investigation included 16\u2005strains of\n                    <jats:italic>Candida<\/jats:italic>\n                    spp, including\n                    <jats:italic>C. albicans<\/jats:italic>\n                    ,\n                    <jats:italic>C. krusei<\/jats:italic>\n                    ,\n                    <jats:italic>C. glabrata<\/jats:italic>\n                    ,\n                    <jats:italic>C. tropicalis<\/jats:italic>\n                    ,\n                    <jats:italic>C. dubliniensis<\/jats:italic>\n                    ,\n                    <jats:italic>C. lusitaniae<\/jats:italic>\n                    ,\n                    <jats:italic>C. utilis<\/jats:italic>\n                    ,\n                    <jats:italic>C. rugosa<\/jats:italic>\n                    ,\n                    <jats:italic>C. guilhermondi<\/jats:italic>\n                    , and\n                    <jats:italic>C. parapsilosis<\/jats:italic>\n                    . Docking analysis predicted affinity between the molecule and all tested targets. MIC and MFC values ranged from 3.9\u2005\u03bcg\/mL (13.54\u2005\u03bcM) to 62.5\u2005\u03bcg\/mL (217.01\u2005\u03bcM), indicating a probable effect on the plasma membrane. The molecule inhibited growth from the first hour of testing. Association with nystatin proved to be indifferent. All concentrations of the molecule reduced fungal biofilm. The compound altered fungal micromorphology. The tested compound exhibited an IC50 of 7.90\u00b10.40\u2005\u03bcg\/mL (27.45\u00b11.42\u2005\u03bcM) for keratinocytes. 4\u2010chlorobenzyl\n                    <jats:italic>p<\/jats:italic>\n                    \u2010coumarate showed strong fungicidal effects, likely through its action on the plasma membrane and alteration of fungal micromorphology, and mildly cytotoxic to human keratinocytes.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202400330","type":"journal-article","created":{"date-parts":[[2024,5,3]],"date-time":"2024-05-03T13:43:15Z","timestamp":1714743795000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Antifungal Activity, Mode of Action, and Cytotoxicity of 4\u2010Chlorobenzyl\n                    <i>p<\/i>\n                    \u2010Coumarate: A Promising New 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