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Meanwhile, their biological activities were evaluated, and the ensuing structure\u2013activity relationships were discussed. The bioassay results showed the substantial antimicrobial efficacy exhibited by most of the compounds. Among them, compound A<jats:sub>24<\/jats:sub> demonstrated a strong efficacy with an EC<jats:sub>50<\/jats:sub> value of 7.8\u2005\u03bcg\/mL in\u2005vitro against the <jats:italic>Xanthomonas oryzae<\/jats:italic> pv. <jats:italic>oryzicola<\/jats:italic> (<jats:italic>Xoc<\/jats:italic>) pathogen, surpassing commercial agents thiodiazole copper (31.8\u2005\u03bcg\/mL) and bismerthiazol (43.3\u2005\u03bcg\/mL). Mechanistic investigations into its anti\u2010Xoc properties revealed that compound A<jats:sub>24<\/jats:sub> operates by increasing the permeability of bacterial cell membranes, inhibiting biofilm formation and cell motility, and inducing morphological changes in bacterial cells. Importantly, in\u2005vivo tests showed its excellent protective and curative effects on rice bacterial leaf streak. Besides, molecular docking showed that the hydrophobic effect and hydrogen\u2010bond interactions are key factors between the binding of A<jats:sub>24<\/jats:sub> and AvrRxo1\u2010ORF1. Therefore, these results suggest the utilization of 1,3,4\u2010thiadiazole derivatives containing sulfonylpiperazine structures as a bacterial biofilm inhibiting agent, warranting further exploration in the realm of agrochemical development.<\/jats:p>","DOI":"10.1002\/cbdv.202400408","type":"journal-article","created":{"date-parts":[[2024,3,5]],"date-time":"2024-03-05T14:36:00Z","timestamp":1709649360000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Synthesis, Structural Characterization, and Biological Activities of 1,3,4\u2010 Thiadiazole Derivatives Containing Sulfonylpiperazine Structures"],"prefix":"10.1002","volume":"21","author":[{"given":"You\u2010hua","family":"Liu","sequence":"first","affiliation":[{"name":"National Key Laboratory of Green Pesticide Key Laboratory of Green Pesticide and Agricultural Bioengineering Ministry of Education Center for R&amp;D of Fine Chemicals of Guizhou University  Guiyang 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