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The need arises to find new therapeutic approaches to combat growing infections. Plants and natural products have been considered a valuable source for discovering new molecules with active ingredients. Diosgenin is a sapogenin found in the families of Leguminosae and Dioscoreaceae, it is obtained mainly from the dioscin saponin through the hydrolysis method, it is a phytochemical that has been highlighted in the treatment of various diseases, as well as in combating microbial resistance. The present study aimed to evaluate the susceptibility of fungal strains to diosgenin, as well as verify the association with the reference drug and evaluate the inhibition of the virulence factor through morphological changes in the yeast state to the filamentous form of hyphae and pseudohyphae in strains of <jats:italic>Candida albicans<\/jats:italic>, <jats:italic>Candida tropicalis<\/jats:italic> and <jats:italic>Candida krusei<\/jats:italic> using the broth microdilution method and microculture technique. Antifungal assays revealed that diosgenin was not able to inhibit the growth of the tested strains. However, it was able to inhibit the fungal dimorphism of the strains evaluated, however further studies are recommended to verify its effectiveness against other virulence factors.<\/jats:p>","DOI":"10.1002\/cbdv.202400444","type":"journal-article","created":{"date-parts":[[2024,4,27]],"date-time":"2024-04-27T01:58:53Z","timestamp":1714183133000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["<i>In vitro<\/i> Evaluation of Fungal Susceptibility and Inhibition of Virulence by Diosgenin"],"prefix":"10.1002","volume":"21","author":[{"given":"Maria","family":"do Socorro Costa","sequence":"first","affiliation":[{"name":"Graduate Program in Biotechnology State University of Cear\u00e1  Fortaleza Cear\u00e1 Brazil"},{"name":"Laboratory of Microbiology and Molecular Biology- LMBM Regional University of Cariri  Crato Cear\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Raquel 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