{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T11:54:19Z","timestamp":1787313259475,"version":"build-2736575974"},"reference-count":46,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,6,14]],"date-time":"2024-06-14T00:00:00Z","timestamp":1718323200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82304879"],"award-info":[{"award-number":["82304879"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Chlorogenic acid (Chl), isochlorogenic acid A (Isochl\u2005A), and isochlorogenic acid B (Isochl\u2005B) are naturally occurring phenolic compounds, which have been shown to exert a regulatory effect on lipid metabolism. However, the mechanism underlying this effect remains unclear. Herein, we investigated the inhibitory effects and underlying mechanisms of these three phenolic compounds on oleic acid (OA)\u2010induced HepG2 cells and high\u2010fat diet (HFD)\u2010fed zebrafish. Lipid accumulation and triacylglycerol levels increased in OA\u2010induced cells, which was attenuated by Chl, Isochl\u2005A, and Isochl\u2005B. Moreover, the levels of malondialdehyde (MDA) and reactive oxygen species (ROS) decreased, while superoxide dismutase (SOD) levels increased by Chl, Isochl\u2005A and Isochl\u2005B treatment. Western blot analysis demonstrated that Chl, Isochl\u2005A and Isochl\u2005B reduced the expression of lipogenesis\u2010related protein, including fatty acid synthase (FAS), acetyl\u2010CoA carboxylase (ACC) and peroxisome proliferator\u2010activated receptor gamma (PPAR\u03b3). Moreover, peroxisome proliferator\u2010activated receptor alpha gamma (PPAR\u03b1) was increased by Chl, Isochl\u2005A, and Isochl\u2005B treatment. In addition, our results indicated that Chl, Isochl\u2005A and Isochl\u2005B decreased lipid profiles and lipid accumulation in HFD\u2010fed zebrafish. Thus, these findings highlight the potential of Chl, Isochl\u2005A, and Isochl\u2005B as effective agents for treating or\/and ameliorating non\u2010alcoholic fatty liver disease (NAFLD).<\/jats:p>","DOI":"10.1002\/cbdv.202400564","type":"journal-article","created":{"date-parts":[[2024,5,6]],"date-time":"2024-05-06T04:19:10Z","timestamp":1714969150000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Chlorogenic Acid and its Isomers Attenuate NAFLD by Mitigating Lipid Accumulation in Oleic Acid\u2010Induced HepG2 Cells and High\u2010Fat Diet\u2010 Fed Zebrafish"],"prefix":"10.1002","volume":"21","author":[{"given":"Fangfang","family":"Tie","sequence":"first","affiliation":[{"name":"Key Laboratory of Tibetan Medicine Research Qinghai Provincial Key Laboratory of Tibetan Medicine Research Northwest Institute of Plateau Biology Chinese Academy of Science  Xining 810008 P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Ding","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tibetan Medicine Research Qinghai Provincial Key Laboratory of Tibetan Medicine Research Northwest Institute of Plateau Biology Chinese Academy of Science  Xining 810008 P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yidan","family":"Gao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tibetan Medicine Research Qinghai Provincial Key Laboratory of Tibetan Medicine Research Northwest Institute of Plateau Biology Chinese Academy of Science  Xining 810008 P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9882-9604","authenticated-orcid":false,"given":"Honglun","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tibetan Medicine Research Qinghai Provincial Key Laboratory of Tibetan Medicine Research Northwest Institute of Plateau Biology Chinese Academy of Science  Xining 810008 P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,6,14]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.31150"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.04.015"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.20466"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2016.02.066"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(98)70599-2"},{"key":"e_1_2_9_6_1","first-page":"25","volume":"11","author":"Fan M.","year":"2019","journal-title":"Nutrients"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2015.00238"},{"key":"e_1_2_9_8_1","first-page":"1","volume":"8","author":"Alves-Bezerra M.","year":"2017","journal-title":"Compr Physiol"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijms.27953"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3350\/cmh.2013.19.3.210"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plipres.2008.08.001"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.4254\/wjh.v7.i8.1012"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.750147"},{"key":"e_1_2_9_14_1","first-page":"36","volume":"25","author":"Sun S. W.","year":"2020","journal-title":"Molecules"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/bcpt.13122"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2020.00582"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2018.10.014"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2021.113951"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3109\/13880209.2015.1075048"},{"key":"e_1_2_9_20_1","first-page":"28","volume":"61","author":"Jackson K. M. P.","year":"2017","journal-title":"Mol. Nutr. Food Res."},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.2147\/DDDT.S228751"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/s11671-018-2720-1"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1089\/jmf.2009.1204"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2006.11.004"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/7514802"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2019.172618"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2017.00929"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2022.1042044"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/4919153"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26165027"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2020.109199"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1039\/D3FO00063J"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2018.02.115"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2016.09.017"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-02325-2"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2016.12.017"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.8b03983"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2014.04.003"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/347645a0"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2014.10.039"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.3945\/jn.110.135210"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.21334"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab255"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22168847"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2022.10.008"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1177\/0023677219869037"}],"container-title":["Chemistry &amp; Biodiversity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.202400564","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,7]],"date-time":"2024-11-07T15:59:13Z","timestamp":1730995153000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbdv.202400564"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,14]]},"references-count":46,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2024,7]]}},"alternative-id":["10.1002\/cbdv.202400564"],"URL":"https:\/\/doi.org\/10.1002\/cbdv.202400564","archive":["Portico"],"relation":{},"ISSN":["1612-1872","1612-1880"],"issn-type":[{"value":"1612-1872","type":"print"},{"value":"1612-1880","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,14]]},"assertion":[{"value":"2024-03-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e202400564"}}