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This work shows relevant data on the antibacterial activity of <jats:italic>Ocotea notata<\/jats:italic> leaves. Dichloromethane fraction (DF) from leaves of <jats:italic>O. notata<\/jats:italic> showed antibacterial activity at the concentration of 512\u2005\u03bcg\/mL against strains of <jats:italic>Staphylococcus<\/jats:italic>. The DF was fractionated to investigate bioactive compounds and yielded six subfractions (DF1\u2010DF6). The DF3, DF5, and DF6 showed bacteriostatic activity, and DF4 showed bactericidal activity at the concentration of 2048\u2005\u03bcg\/mL and additive effect when combined with the antibiotic oxacillin. The flavonoids miquelianin (<jats:bold>1<\/jats:bold>), isoquercitrin (<jats:bold>2<\/jats:bold>), reynoutrin (<jats:bold>3<\/jats:bold>), guaijaverin (<jats:bold>4<\/jats:bold>), and afzelin (<jats:bold>5<\/jats:bold>) were isolated from the DF4 and characterized via NMR and HPLC\u2010DAD\u2010MS\/MS analyses. Flavonoids <jats:bold>1<\/jats:bold>\u2010<jats:bold>5<\/jats:bold>, especially reynoutrin (<jats:bold>3<\/jats:bold>), seem to be responsible for the DF4 antibacterial activity. Reynoutrin showed activity against <jats:italic>Staphylococcus epidermidis<\/jats:italic> ATCC 12228 and <jats:italic>S. aureus<\/jats:italic> methicillin\u2010resistant ATCC 33591. This is the first report regarding the reynoutrin antibacterial activity and the first description of the flavonoid guaijaverin for <jats:italic>O. notata<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/cbdv.202400677","type":"journal-article","created":{"date-parts":[[2024,10,8]],"date-time":"2024-10-08T17:08:47Z","timestamp":1728407327000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Antibacterial Activity of Glycosylated Flavonoids Isolated from <i>Ocotea notata<\/i> Leaves"],"prefix":"10.1002","volume":"22","author":[{"given":"Isabela Francisca","family":"de Jesus Borges Costa","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Produtos Bioativos Instituto de Ci\u00eancias Farmac\u00eauticas Universidade Federal do Rio de Janeiro  Campus Maca\u00e9, RJ Brazil"},{"name":"Laborat\u00f3rio de Produtos Naturais e Ensaios Biol\u00f3gicos Faculdade de Farm\u00e1cia Universidade Federal do Rio de Janeiro  Rio de Janeiro, RJ Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o Vitor","family":"Rocha Reis","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Produtos Bioativos Instituto de Ci\u00eancias Farmac\u00eauticas Universidade Federal do Rio de Janeiro  Campus Maca\u00e9, RJ Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatiana","family":"Ungaretti Paleo Konno","sequence":"additional","affiliation":[{"name":"N\u00facleo de Estudos em Ecologia e Desenvolvimento S\u00f3cio-Ambiental de Maca\u00e9 Universidade Federal do Rio de Janeiro  Maca\u00e9, RJ Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luzineide","family":"Wanderley Tinoco","sequence":"additional","affiliation":[{"name":"Instituto de Pesquisa de Produtos Naturais Walter Mors Centro de Ci\u00eancias da Sa\u00fade Universidade Federal do Rio de Janeiro  RJ Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaft","family":"Correa Pinto","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Produtos Bioativos Instituto de Ci\u00eancias Farmac\u00eauticas Universidade Federal do Rio de Janeiro  Campus Maca\u00e9, RJ Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivana","family":"Correa Ramos Leal","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Produtos Naturais e Ensaios Biol\u00f3gicos Faculdade de Farm\u00e1cia Universidade Federal do Rio de Janeiro  Rio de Janeiro, RJ Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0155-3240","authenticated-orcid":false,"given":"Michelle","family":"Fraz\u00e3o Muzitano","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Produtos Bioativos Instituto de Ci\u00eancias Farmac\u00eauticas Universidade Federal do Rio de Janeiro  Campus Maca\u00e9, RJ Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,11,9]]},"reference":[{"key":"e_1_2_10_1_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.17.1.218-234.2004"},{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2013.02.007"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/003335490712200205"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.mm6809e1"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/bjaceaccp\/mki006"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines10051121"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2004.08.007"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2011.09.030"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3934\/microbiol.2018.3.482"},{"key":"e_1_2_10_10_1","doi-asserted-by":"crossref","unstructured":"Sharma S Mohler J Mahajan SD Schwartz SA Bruggemann L Aalinkeel R. 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