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Among the metabolites found in this species are the phytosterols beta\u2010sitosterol (\u03b2\u2010SIT) and stigmasterol (STIG). The objective of the study was to evaluate the anti\u2010inflammatory and toxicity activities of the hexane partition of <jats:italic>P. aculeata<\/jats:italic> (PHEX), as well as \u03b2\u2010SIT and STIG. PHEX was prepared and the phytosterols were quantified. In terms of toxicity against L929 fibroblast cells, PHEX showed toxicity up to 200\u2005\u03bcg\/mL; STIG and \u03b2\u2010SIT showed toxicity up to 25\u2005\u03bcg\/mL. PHEX inhibited 66\u2009% of nitric oxide radicals, while STIG and \u03b2\u2010SIT inhibited 33.73\u2009% and 34.94\u2009%, respectively. In an anti\u2010inflammatory test against <jats:italic>Zophobas morio<\/jats:italic> larvae, all samples significantly reduced hemocyte levels. Additionally, the LD50 values were calculated: 229.6\u2005mg\/kg for PHEX, 101.5\u2005mg\/kg for STIG, and 103.8\u2005mg\/kg for \u03b2\u2010SIT. In conclusion, the study indicates that the phytosterols present in PHEX may contribute to its anti\u2010inflammatory activity.<\/jats:p>","DOI":"10.1002\/cbdv.202400686","type":"journal-article","created":{"date-parts":[[2024,6,25]],"date-time":"2024-06-25T15:04:39Z","timestamp":1719327879000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Anti\u2010Inflammatory Activity and Acute Toxicity Of <i>Pereskia aculeata,<\/i> In <i>Zophobas morio<\/i> Larvae"],"prefix":"10.1002","volume":"21","author":[{"given":"Dominique Mesquita","family":"e Silva","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Sciences Faculty of Pharmacy Federal University of Juiz de Fora  Juiz de Fora 36036-900 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-9586-5611","authenticated-orcid":false,"given":"Luiza Gabrielle Assun\u00e7\u00e3o","family":"Nunes","sequence":"additional","affiliation":[{"name":"Department of Medicines Faculty of Pharmacy Federal University of Bahia  Salvador 40170-115 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nat\u00e1lia","family":"Prado da Silva","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences Faculty of Pharmacy Federal University of Juiz de Fora  Juiz de Fora 36036-900 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro Henrique Santos","family":"de Freitas","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Institute of Biological Sciences Federal University of Juiz de Fora  Juiz de Fora 36036-900 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elita","family":"Scio","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Institute of Biological Sciences Federal University of Juiz de Fora  Juiz de Fora 36036-900 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guilherme Diniz","family":"Tavares","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences Faculty of Pharmacy Federal University of Juiz de Fora  Juiz de Fora 36036-900 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Izabel","family":"Almeida Alves","sequence":"additional","affiliation":[{"name":"Department of Medicines Faculty of Pharmacy Federal University of Bahia  Salvador 40170-115 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana","family":"de Carvalho da Costa","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences Faculty of Pharmacy Federal University of Juiz de Fora  Juiz de Fora 36036-900 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,8,14]]},"reference":[{"key":"e_1_2_9_1_1","first-page":"1748","volume":"4","author":"Freitas P. 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