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This study focused on exploring the immunomodulatory potential of chrysin on NK\u201092 and Jurkat\u2010T cells targeting breast cancer cells (BCC). Chrysin leads to activation of NK\u201092 and T cells facilitated by the addition of human recombinant IL\u20102 and PHA\u2212M. The anti\u2010cancer efficacy of chrysin on these immune cells was evaluated in a co\u2010culture setup with EGF\u2010stimulated MCF\u20107 and MDA\u2010MB\u2010231 cells. Findings revealed that chrysin notably increased the cytotoxicity of NK\u201092 and T cells towards MCF\u20107 and MDA\u2010MB\u2010231 cells, with the most significant impact observed on MCF\u20107 cells (20\u2009%). The activation of NK\u201092 cells, marked by increased IFN\u2010\u03b3 production and CD56 expression, correlated with enhanced secretion of cytokines. Additionally, the activation of these cells against BCC was linked with elevated levels of granzyme\u2010B, TNF\u2010\u03b1, and nitric oxide (NO). Similarly, the cytotoxic activation of Jurkat\u2010T cells against BCC was characterized by increased production of granzyme\u2010B, IL\u20102, and IFN\u2010\u03b3. Consequently, these results support the hypothesis that chrysin significantly contributes to the activation and functional enhancement of NK\u201092 and T\u2010cells against two distinct BCC lines.<\/jats:p>","DOI":"10.1002\/cbdv.202400806","type":"journal-article","created":{"date-parts":[[2024,7,11]],"date-time":"2024-07-11T17:25:26Z","timestamp":1720718726000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Chrysin Enhances Anti\u2010Cancer Activity of Jurkat T Cell and NK\u201092 Cells Against Human Breast Cancer Cell Lines"],"prefix":"10.1002","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0760-497X","authenticated-orcid":false,"given":"Ezgi","family":"Durmus","sequence":"first","affiliation":[{"name":"Department of Medical Biochemistry Institute of Health Sciences Bezmialem Vakif University  34093 Istanbul Turkey"},{"name":"Department of Medical Biochemistry Faculty of Medicine Bezmialem 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