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This study was undertaken to investigate extraction, purification, structural elucidation, and the potential anti\u2010cancer effects of <jats:italic>Pleurotus ostreatus<\/jats:italic> polysaccharide (POP). The anti\u2010cancer activities were performed on the Ehrlich Ascites Carcinoma Cell Line. The results demonstrated that the MW of POP was154649.8\u2005Da with homopolysaccharide composed of D\u2010glucose units, featuring (1\u21926)\u2010\u03b1\u2010D\u2010Glcp backbone with O\u20106 branches and T\u2010\u03b1\u2010D\u2010Glcp terminations. and the yield was 6.27\u2009%. The antitumor activity assessment demonstrated significant cytotoxicity of POP against Ehrlich Ascites Carcinoma (EAC) cells, with an IC<jats:sub>50<\/jats:sub> of 121.801\u2005\u03bcg\u2009mL, supported by LDH release analysis. POP inhibited cell migration, invasion, and colony formation, indicating its potential as an anti\u2010cancer agent. POP elicited the apoptotic activity with the upregulation of Caspase\u20109 and Bax, and downregulation of Bcl\u20102. The DNA fragmentation assay further confirmed apoptosis\u2010mediated DNA degradations. Additionally, POP\u2010induced cell cycle arrest at the G0\/G1 phase, by altering the expression of p53, Cyclin D, and Cdk4 proteins. So, <jats:italic>Pleurotus ostreatus<\/jats:italic> polysaccharide (POP) showed significant cytotoxicity on Ehrlich Ascites Carcinoma cells, indicating potential as an anti\u2010cancer agent.<\/jats:p>","DOI":"10.1002\/cbdv.202400897","type":"journal-article","created":{"date-parts":[[2024,7,6]],"date-time":"2024-07-06T14:14:23Z","timestamp":1720275263000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Investigating the Anticancer Effects of <i>Pleurotus ostreatus<\/i> Polysaccharide on G0\/G1 Cell Cycle Arrest and Apoptosis in Ehrlich Ascites Carcinoma Cells"],"prefix":"10.1002","volume":"21","author":[{"given":"Md","family":"Moyen Uddin PK","sequence":"first","affiliation":[{"name":"Institute of Biological Sciences Rajshahi University  Rajshahi-6205 Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane","family":"O'Sullivan","sequence":"additional","affiliation":[{"name":"Department of Anaesthesiology and Critical Care Tallaght University Hospital  Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad","family":"Sayful Islam","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Mawlana Bhashani Science and Technology University  Tangail-1902 Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad","family":"Shahangir Biswas","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biotechnology Khwaja Yunus Ali University  Sirajganj 6751 Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lubatul","family":"Arbia","sequence":"additional","affiliation":[{"name":"Department of Biochemistry &amp; Molecular Biology Primeasia University  Dhaka-1213 Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rumana","family":"Pervin","sequence":"additional","affiliation":[{"name":"Department of Biochemistry &amp; Molecular Biology University of Rajshahi  Rajshahi-6205 Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matiar","family":"Rahman","sequence":"additional","affiliation":[{"name":"Department of Biochemistry &amp; Molecular Biology University of Rajshahi  Rajshahi-6205 Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,8,26]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.20107"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/md12094898"},{"issue":"1","key":"e_1_2_9_3_1","first-page":"26","volume":"15","author":"Hosseini B.-A.","year":"2015","journal-title":"Bosn. 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