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This study evaluated the antioxidant and cytotoxic potentials of its ethanolic and methanolic extracts, both <jats:italic>in\u2005vitro<\/jats:italic> and <jats:italic>in\u2005vivo<\/jats:italic>, while also analyzing their chemical profiles. HPLC analysis identified key compounds, including gallic acid, caffeic acid, caffeine, coumarin, rutin, catechin, ferulic acid, and quercetin. Chronic toxicity assessments confirmed the safety of the extracts, with no adverse effects on animal health, particularly in liver histopathology. Cytotoxicity results indicated reduced splenocyte viability at the highest concentrations. The extracts exhibited significant antioxidant activity in DPPH<jats:sup>\u2022<\/jats:sup>, FRP, and phosphomolybdate assays, demonstrating their effectiveness as antiradical agents. In vivo antioxidant results showed a reduction in lipid peroxidation levels in serum and liver, highlighting the extracts\u2019 ability to mitigate oxidative stress. Additionally, the extracts provided protection against H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010induced erythrocyte hemolysis and modulated NO production in peritoneal macrophages. These findings underscore the therapeutic potential of <jats:italic>A. melegueta<\/jats:italic> extracts, suggesting their promise in developing preventive strategies for oxidative stress\u2010related chronic diseases.<\/jats:p>","DOI":"10.1002\/cbdv.202400942","type":"journal-article","created":{"date-parts":[[2024,9,14]],"date-time":"2024-09-14T02:14:30Z","timestamp":1726280070000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["<i>Aframomum Melegueta<\/i>: Evaluation of Chronic Toxicity, HPLC Profiling, and <i>In Vitro<\/i>\/<i>In Vivo<\/i> Antioxidant Assessment of Seeds Extracts"],"prefix":"10.1002","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0151-9627","authenticated-orcid":false,"given":"Latif","family":"Mounia","sequence":"first","affiliation":[{"name":"Immunology and Biodiversity Laboratory Faculty of Sciences Ain Chock Hassan II University  Casablanca Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7562-5222","authenticated-orcid":false,"given":"Elkoraichi","family":"Ismail","sequence":"additional","affiliation":[{"name":"Immunology and Biodiversity Laboratory Faculty of Sciences Ain Chock Hassan II University  Casablanca Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6014-3618","authenticated-orcid":false,"given":"El Faqer","family":"Othman","sequence":"additional","affiliation":[{"name":"Immunology and Biodiversity Laboratory Faculty of Sciences Ain Chock Hassan II University  Casablanca Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1375-4111","authenticated-orcid":false,"given":"Wahnou","family":"Hicham","sequence":"additional","affiliation":[{"name":"Immunology and Biodiversity Laboratory Faculty of Sciences Ain Chock Hassan II University  Casablanca Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1749-7178","authenticated-orcid":false,"given":"Elaaj","family":"Rajaa","sequence":"additional","affiliation":[{"name":"Immunology and Biodiversity Laboratory Faculty of Sciences Ain Chock Hassan II University  Casablanca Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0653-2166","authenticated-orcid":false,"given":"Mtairag","family":"El Mostafa","sequence":"additional","affiliation":[{"name":"Immunology and Biodiversity Laboratory Faculty of Sciences Ain Chock Hassan II University  Casablanca Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5815-1579","authenticated-orcid":false,"given":"Oudghiri","family":"Mounia","sequence":"additional","affiliation":[{"name":"Immunology and Biodiversity Laboratory Faculty of Sciences Ain Chock Hassan II University  Casablanca Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6232-4893","authenticated-orcid":false,"given":"Rais","family":"Samira","sequence":"additional","affiliation":[{"name":"Immunology and Biodiversity Laboratory Faculty of Sciences Ain Chock Hassan II University  Casablanca Morocco"},{"name":"Department of Biology Faculty of Sciences Ben M'Sick Hassan II University  Casablanca Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,31]]},"reference":[{"key":"e_1_2_11_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2013.06.010"},{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pnpbp.2013.02.015"},{"key":"e_1_2_11_3_1","first-page":"1","volume":"2017","author":"Pizzino G.","year":"2017","journal-title":"Oxid. 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