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This study aims to investigate the antiviral activity of BBR against rotavirus and evaluate its cytotoxicity and pharmacological efficacies, including antioxidant and anti\u2010inflammatory activities <jats:italic>in\u2005vitro<\/jats:italic>. Using ultraviolet\u2010visible absorption spectroscopy, the saturation concentration of BBR was determined as 2261\u2005\u03bcg\/mL, indicating that BBR is a poor water\u2010soluble compound. The inhibition rate of nitric oxide (NO) production of BBR solution at a concentration of 283\u2005\u03bcg\/mL was similar to that of Cardamonin 0.3\u2005\u03bcM with a cell viability of 92.46\u00b10.35\u2009%, revealing the anti\u2010inflammatory activity of BBR. The cytotoxicity of the BBR solution depended on its concentration, whereby the 50\u2009% cytotoxicity concentration (CC<jats:sub>50<\/jats:sub>) of BBR after 96\u2005h exposure was 664\u2005\u03bcg\/mL. Investigation of cytopathic effects (CPEs) of MA104 cells treated with BBR and BBR\u2010incubated rotavirus indicates that BBR could effectively inhibit the replication of rotavirus. CPEs were not observed in the cells inoculated with rotavirus (100TCID<jats:sub>50<\/jats:sub>) which was pre\u2010incubated with BBR for 96\u2005hours at a BBR concentration of 283\u2005\u03bcg\/mL. Therefore, the study provides reliable results to demonstrate the ability of BBR to inhibit the replication of rotavirus.<\/jats:p>","DOI":"10.1002\/cbdv.202400986","type":"journal-article","created":{"date-parts":[[2024,10,14]],"date-time":"2024-10-14T14:04:44Z","timestamp":1728914684000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["<i>In Vitro<\/i> Inhibitory Effect of Berberine Against Rotavirus"],"prefix":"10.1002","volume":"22","author":[{"given":"Hue Thi","family":"Nguyen","sequence":"first","affiliation":[{"name":"Phenikaa University Nano Institute (PHENA) Phenikaa University  Hanoi 12116 Vietnam"},{"name":"Faculty of Biomedical Sciences Phenikaa University  Hanoi 12116 Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thi Nguyen","family":"Hoa\u2010Tran","sequence":"additional","affiliation":[{"name":"Department of Virology National Institute of Hygiene and Epidemiology  Hanoi Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2812-3937","authenticated-orcid":false,"given":"Huy Quang","family":"Tran","sequence":"additional","affiliation":[{"name":"Phenikaa University Nano Institute (PHENA) Phenikaa University  Hanoi 12116 Vietnam"},{"name":"Faculty of Biomedical Sciences Phenikaa University  Hanoi 12116 Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7239-8458","authenticated-orcid":false,"given":"Thuy Thi Thu","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Phenikaa University Nano Institute (PHENA) Phenikaa University  Hanoi 12116 Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,11,12]]},"reference":[{"key":"e_1_2_10_1_1","first-page":"115","volume":"7","author":"Prajwala B.","year":"2020","journal-title":"Eur. 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