{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T16:33:40Z","timestamp":1790699620050,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,8,16]],"date-time":"2024-08-16T00:00:00Z","timestamp":1723766400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Research interest in examining <jats:italic>Elaeagnus angustifolia<\/jats:italic>\u2019s potential as a source of anti\u2010inflammatory and antioxidant agents has grown as a result of the plant's endorsement as a rich source of bioactive chemicals with promising anti\u2010inflammatory and antioxidant activity. In this study, zinc oxide (Fe0.25\u2010ZnO) bimetallic nanoparticles (<jats:italic>E.ang<\/jats:italic>\u2010Fe0.25\u2010ZnO NPs) were synthesized using an aqueous extract of <jats:italic>Elaeagnus angustifolia<\/jats:italic>. Synthesized Fe0.25\u2010ZnO nanoparticles were characterized by FTIR and XRD. The anti\u2010inflammatory and antioxidant activities were investigated in LPS\u2010stimulated RAW 264.7 macrophages using RT\u2010PCR and ELISA techniques for antioxidant\u2010 and inflammation\u2010related genes. The concentration of 39.6\u2005\u03bcg\/ml of <jats:italic>E.ang<\/jats:italic>\u2010Fe0.25\u2010ZnO NPs demonstrated a significant anti\u2010inflammatory activity by suppressing the mRNA levels of TNF\u2010\u03b1 and IL\u20106 by 88.3\u2009%\u00b11.9 and 93.6\u2009%\u00b10.1, respectively, compared to LPS\u2010stimulated cells. This was confirmed by the significant reduction of TNF\u2010\u03b1 and IL\u20106 secretion levels from 95.2 and 495.6\u2005pg\/ml in LPS\u2010stimulated cells to 5.6 and 26.5\u2005pg\/ml in <jats:italic>E.ang<\/jats:italic>\u2010Fe0.25\u2010ZnO treated group. In addition, <jats:italic>E.ang<\/jats:italic>\u2010Fe0.25\u2010ZnO NPs nanoparticles treatment significantly enhanced the expression of antioxidant\u2010related genes, SOD and CAT. Together, our results proved that phyto\u2010mediated Fe0.25\u2010ZnO nanoparticles using <jats:italic>Elaeagnus angustifolia<\/jats:italic> have great potential in biomedical applications such as anti\u2010inflammatory and antioxidant.<\/jats:p>","DOI":"10.1002\/cbdv.202401060","type":"journal-article","created":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T12:04:08Z","timestamp":1719835448000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Anti\u2010Inflammatory and Antioxidant Potential of Green Synthesized Iron Zinc Oxide (Fe0.25\u2010ZnO) Nanoparticles of the Elaeagnus angustifolia"],"prefix":"10.1002","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1191-2905","authenticated-orcid":false,"given":"Amer","family":"Imraish","sequence":"first","affiliation":[{"name":"Department of Biological Sciences School of Science The University of Jordan  Queen Rania Al-Abdullah Street Amman 11942 Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tuqa Abu","family":"Thiab","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences School of Science The University of Jordan  Queen Rania Al-Abdullah Street Amman 11942 Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malek","family":"Zihlif","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Faculty of Medicine The University of Jordan  Amman Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Afnan","family":"Al\u2010Hunaiti","sequence":"additional","affiliation":[{"name":"Department of Chemistry School of Science The University of Jordan  Queen Rania Al-Abdullah Street Amman 11942 Jordan."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,8,16]]},"reference":[{"issue":"1","key":"e_1_2_6_1_1","first-page":"1","volume":"7","author":"Musa N. 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