{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T07:36:42Z","timestamp":1781336202694,"version":"3.54.1"},"reference-count":39,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,7,17]],"date-time":"2024-07-17T00:00:00Z","timestamp":1721174400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["42076099"],"award-info":[{"award-number":["42076099"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Hepatitis B Virus (HBV) infection is a global public health challenge that seriously endangers human health. Soft coral, as a major source of terpenoids, contains many structurally novel and highly bioactive compounds. Sixteen cembranoids (<jats:bold>1<\/jats:bold>\u2010<jats:bold>16<\/jats:bold>), including a new one named sinupedunol B (<jats:bold>16<\/jats:bold>), were isolated from the South China Sea Soft coral <jats:italic>Sinularia pedunculata<\/jats:italic>. The structure of the sinupedunol B (<jats:bold>16<\/jats:bold>) was determined through a combination of spectroscopic analysis and X\u2010ray single\u2010crystal diffraction. In this study, cembranoids isolated from <jats:italic>Sinularia pedunculata<\/jats:italic> were found of anti\u2010HBV activity for the first time. Among them, flexilarin D (<jats:bold>6<\/jats:bold>) showed significant anti\u2010HBV activity with an IC<jats:sub>50<\/jats:sub> value of 5.57\u2005<jats:italic>\u03bc<\/jats:italic>M without cytotoxicity. We then analyzed the structure\u2010activity relationship (SAR). Furthermore, it is demonstrated that flexilarin D (<jats:bold>6<\/jats:bold>) can accelerate the formation of capsid, inhibit HBeAg, HBV core particle DNA, HBV total RNA and pregenomic RNA in a dose dependent manner. We also confirmed the anti\u2010HBV activity of <jats:bold>6<\/jats:bold> in HepG2\u2010NTCP infection system. Finally, we demonstrated the anti\u2010HBV mechanism of these compounds by inhibiting the ENI\/Xp enhancer\/promoter.<\/jats:p>","DOI":"10.1002\/cbdv.202401146","type":"journal-article","created":{"date-parts":[[2024,5,22]],"date-time":"2024-05-22T02:58:24Z","timestamp":1716346704000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Anti\u2010HBV Activities of Cembranoids from the South China Sea Soft Coral <i>Sinularia pedunculata<\/i> and Their Structure Activity Relationship"],"prefix":"10.1002","volume":"21","author":[{"given":"Chao","family":"Huang","sequence":"first","affiliation":[{"name":"Key Laboratory of Medical Molecular Virology (MOE\/NHC) Research Unit of Cure of Chronic Hepatitis B Virus Infection (CAMS) Shanghai Frontiers Science Center of Pathogenic Microbes and Infection School of Basic Medical Sciences Shanghai Medical College Fudan University  Shanghai 200032 China"},{"name":"State Key Laboratory of Chemical Biology Shanghai Institute of Materia Medica Chinese Academy of Sciences  Shanghai 201203 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Jin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Chemical Biology Shanghai Institute of Materia Medica Chinese Academy of Sciences  Shanghai 201203 China"},{"name":"Shandong Laboratory of Yantai Drug Discovery Bohai Rim Advanced Research Institute for Drug Discovery  Yantai Shandong 264117 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruo\u2010Nan","family":"Sun","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Chemical Biology Shanghai Institute of Materia Medica Chinese Academy of Sciences  Shanghai 201203 China"},{"name":"Shandong Laboratory of Yantai Drug Discovery Bohai Rim Advanced Research Institute for Drug Discovery  Yantai Shandong 264117 China"},{"name":"University of Chinese Academy of Sciences  No. 19\u2005A Yuquan Road Beijing 100049 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kong\u2010Ying","family":"Hu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Medical Molecular Virology (MOE\/NHC) Research Unit of Cure of Chronic Hepatitis B Virus Infection (CAMS) Shanghai Frontiers Science Center of Pathogenic Microbes and Infection School of Basic Medical Sciences Shanghai Medical College Fudan University  Shanghai 200032 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li\u2010Gong","family":"Yao","sequence":"additional","affiliation":[{"name":"Shandong Laboratory of Yantai Drug Discovery Bohai Rim Advanced Research Institute for Drug Discovery  Yantai Shandong 264117 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue\u2010Wei","family":"Guo","sequence":"additional","affiliation":[{"name":"Shandong Laboratory of Yantai Drug Discovery Bohai Rim Advanced Research Institute for Drug Discovery  Yantai Shandong 264117 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng\u2010Hong","family":"Yuan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Medical Molecular Virology (MOE\/NHC) Research Unit of Cure of Chronic Hepatitis B Virus Infection (CAMS) Shanghai Frontiers Science Center of Pathogenic Microbes and Infection School of Basic Medical Sciences Shanghai Medical College Fudan University  Shanghai 200032 China"},{"name":"Shanghai Institute of Infectious Disease and Biosecurity  Shanghai 200032 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7919-9726","authenticated-orcid":false,"given":"Xu\u2010Wen","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Chemical Biology Shanghai Institute of Materia Medica Chinese Academy of Sciences  Shanghai 201203 China"},{"name":"Shandong Laboratory of Yantai Drug Discovery Bohai Rim Advanced Research Institute for Drug Discovery  Yantai Shandong 264117 China"},{"name":"University of Chinese Academy of Sciences  No. 19\u2005A Yuquan Road Beijing 100049 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,7,17]]},"reference":[{"key":"e_1_2_12_1_1","unstructured":"World Health Organization. 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