{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:35:43Z","timestamp":1786977343076,"version":"build-2736575974"},"reference-count":42,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2024,10,26]],"date-time":"2024-10-26T00:00:00Z","timestamp":1729900800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2024,10,26]],"date-time":"2024-10-26T00:00:00Z","timestamp":1729900800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100012046","name":"Vietnam Academy of Science and Technology","doi-asserted-by":"publisher","award":["THTEXS.05\/22-25"],"award-info":[{"award-number":["THTEXS.05\/22-25"]}],"id":[{"id":"10.13039\/100012046","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Piper longum<\/jats:italic>\n                    L. (PL) is considered one of the most important species traditionally used for treating various ailments and has indicated the presence of alkaloids, flavonoids, and steroids. In this study, we isolated the chemical compounds of\n                    <jats:italic>PL leaves, and measured NO<\/jats:italic>\n                    , IL\u20106, iNOS, as well as COX\u20102 protein levels. In addition, molecular docking analysis was used to further understand the anti\u2010inflammation effect of the compounds. We identified one new alkaloid named piperlongumine A (\n                    <jats:bold>1<\/jats:bold>\n                    ) with ten known compounds (\n                    <jats:bold>2<\/jats:bold>\n                    \u2010\n                    <jats:bold>11<\/jats:bold>\n                    )\n                    <jats:italic>\n                      . The new compound (\n                      <jats:bold>1<\/jats:bold>\n                      ) and two other alkaloids\n                    <\/jats:italic>\n                    2\n                    <jats:italic>E<\/jats:italic>\n                    )\u20103\u2010(4\u2010hydroxy\u20103\u2010methoxyphenyl)\u20101\u2010(pyrrol\u20101\u2010yl) propanone (\n                    <jats:bold>7<\/jats:bold>\n                    ) and piperchabamide A (\n                    <jats:bold>8<\/jats:bold>\n                    )\n                    <jats:italic>significantly reduced<\/jats:italic>\n                    NO production in LPS\u2010stimulated RAW 264.7 cells with the IC\n                    <jats:sub>50<\/jats:sub>\n                    values of 0.97\u00b10.05\u2005\u03bcM, 0.91\u00b10.07\u2005\u03bcM, 1.63\u00b10.14\u2005\u03bcM, respectively. Moreover, at concentration of 2\u2005\u03bcM, compound\n                    <jats:bold>1<\/jats:bold>\n                    inhibited approximately 98\u00b10.64\u2009% of IL\u20106 secretion, and decreased iNOS and COX\u20102 protein level by about 96 and 19 folds compared to LPS treatment alone, respectively. Furthermore, compounds\n                    <jats:bold>1<\/jats:bold>\n                    ,\n                    <jats:bold>7<\/jats:bold>\n                    , and\n                    <jats:bold>8<\/jats:bold>\n                    were predicted to bind and inhibit IL\u20106, TNF\u2010\u03b1, and iNOS, with compound\n                    <jats:bold>1<\/jats:bold>\n                    showing the highest binding energy of \u22127.09\u2005kcal\/mol. This study provides new insights for potential anti\u2010inflammatory drug design and warrants further investigation.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202401224","type":"journal-article","created":{"date-parts":[[2024,8,16]],"date-time":"2024-08-16T05:49:28Z","timestamp":1723787368000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Alkaloids from\n                    <i>Piper longum<\/i>\n                    L and their Anti\u2010inflammatory Properties"],"prefix":"10.1002","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1726-5673","authenticated-orcid":false,"given":"Thi Thu","family":"Phuong Tran","sequence":"first","affiliation":[{"name":"University of Science and Technology of Hanoi (USTH) Vietnam Academy of Science and Technology (VAST)  18 Hoang Quoc Viet Cau Giay, Hanoi 100000 Viet Nam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1161-6498","authenticated-orcid":false,"given":"Nguyen Xuan","family":"Nhiem","sequence":"additional","affiliation":[{"name":"Institute of Marine Biochemistry VAST  18 Hoang Quoc Viet Cau Giay, Hanoi 100000 Viet Nam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4531-7223","authenticated-orcid":false,"given":"Hai","family":"Pham\u2010The","sequence":"additional","affiliation":[{"name":"University of Science and Technology of Hanoi (USTH) Vietnam Academy of Science and Technology (VAST)  18 Hoang Quoc Viet Cau Giay, Hanoi 100000 Viet Nam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uyen Thi Tu","family":"Phan","sequence":"additional","affiliation":[{"name":"University of Science and Technology of Hanoi (USTH) Vietnam Academy of Science and Technology (VAST)  18 Hoang Quoc Viet Cau Giay, Hanoi 100000 Viet Nam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Le Thanh","family":"Huong","sequence":"additional","affiliation":[{"name":"University of Science and Technology of Hanoi (USTH) Vietnam Academy of Science and Technology (VAST)  18 Hoang Quoc Viet Cau Giay, Hanoi 100000 Viet Nam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4470-8321","authenticated-orcid":false,"given":"Hai Dang","family":"Nguyen","sequence":"additional","affiliation":[{"name":"University of Science and Technology of Hanoi (USTH) Vietnam Academy of Science and Technology (VAST)  18 Hoang Quoc Viet Cau Giay, Hanoi 100000 Viet Nam"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,26]]},"reference":[{"key":"e_1_2_6_1_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/6027305"},{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.06.036"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules16042802"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules16075754"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules15031811"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/md18030147"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0102-695X2006000100020"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.4103\/0973-7847.91102"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2019.00001"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24071364"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2003.10.016"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.7649"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-4039(01)99798-9"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-4020(01)82575-8"},{"key":"e_1_2_6_15_1","first-page":"863","volume":"4","author":"Deepthi Swapna P. 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