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The WSF obtained using a Clevenger\u2010type apparatus was further divided into two equal parts, one each for in\u2010vivo toxicity evaluation and quality assessments, respectively. The proportions of major phenylpropanoid identified as <jats:italic>meta<\/jats:italic>\u2010eugenol in the WSF were found in the range of 42.8\u201357.9\u2009%, which was substantially in higher proportion as compared to essential oil (20.9\u201323.0\u2009%). Based on sub\u2010acute oral toxicity data, WSF has not shown any adverse effect with levels as high as 500\u2005\u03bcL\/25\u2005g body weight in Swiss albino mice. Besides, the WSF also exhibited a maximum reduction in bacterial load in mice infected with <jats:italic>Salmonella<\/jats:italic> Typhimurium in a dose\u2010dependent manner. We have shown the biological potential of basil water\u2010soluble fraction as an effective bacterial load\u2010suppressing agent for the prevention of <jats:italic>Salmonella<\/jats:italic> infections in animal model.<\/jats:p>","DOI":"10.1002\/cbdv.202401284","type":"journal-article","created":{"date-parts":[[2024,11,6]],"date-time":"2024-11-06T06:19:55Z","timestamp":1730873995000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Unraveling Water\u2010Soluble Constituents of Basil (<i>Ocimum basilicum<\/i> L.) in Relation to Their Toxicity and Anti\u2010Typhoidal Activity in Mouse Models"],"prefix":"10.1002","volume":"22","author":[{"given":"Kamlesh","family":"Yadav","sequence":"first","affiliation":[{"name":"Laboratory of Aromatic Plants and Chiral Separation Phytochemistry Division CSIR-Central Institute of Medicinal and Aromatic Plants  Lucknow, Uttar Pradesh 226015 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shubham","family":"Srivastava","sequence":"additional","affiliation":[{"name":"Laboratory of Aromatic Plants and Chiral Separation Phytochemistry Division CSIR-Central Institute of Medicinal and Aromatic Plants  Lucknow, Uttar Pradesh 226015 India"},{"name":"Academy of Scientific and Innovative Research (AcSIR)  Ghaziabad 201002 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yatish","family":"Pant","sequence":"additional","affiliation":[{"name":"Laboratory of Aromatic Plants and Chiral Separation Phytochemistry Division CSIR-Central Institute of Medicinal and Aromatic Plants  Lucknow, Uttar Pradesh 226015 India"},{"name":"Academy of Scientific and Innovative Research (AcSIR)  Ghaziabad 201002 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raj K","family":"Lal","sequence":"additional","affiliation":[{"name":"Plant Breeding and Genetic Resources Conservation Division CSIR-Central Institute of Medicinal and Aromatic Plants  Lucknow, Uttar Pradesh 226015 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anand","family":"Mishra","sequence":"additional","affiliation":[{"name":"Plant Breeding and Genetic Resources Conservation Division CSIR-Central Institute of Medicinal and Aromatic Plants  Lucknow, Uttar Pradesh 226015 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laldingngheti","family":"Bawitlung","sequence":"additional","affiliation":[{"name":"Bio-prospection and Product Development Division CSIR-Central Institute of Medicinal and Aromatic Plants  Lucknow, Uttar Pradesh 226015 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deepika","family":"Srivastava","sequence":"additional","affiliation":[{"name":"Bio-prospection and Product Development Division CSIR-Central Institute of Medicinal and Aromatic Plants  Lucknow, Uttar Pradesh 226015 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anirban","family":"Pal","sequence":"additional","affiliation":[{"name":"Academy of Scientific and Innovative Research (AcSIR)  Ghaziabad 201002 India"},{"name":"Bio-prospection and Product Development Division CSIR-Central Institute of Medicinal and Aromatic Plants  Lucknow, Uttar Pradesh 226015 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9802-0162","authenticated-orcid":false,"given":"C. 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