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This investigation, focusing on identifying potential antidiabetic compounds from the endangered plant species <jats:italic>Vepris glandulosa<\/jats:italic>, led to the isolation of two known alkaloids, choisyine acetate (<jats:bold>1<\/jats:bold>) and choisyine (<jats:bold>2<\/jats:bold>). The study established the in\u2005vitro inhibitory activities and in silico molecular interaction of the two alkaloids with \u03b1\u2010amylase based on IC<jats:sub>50<\/jats:sub> values, Linewaever\u2010Burk\/Dixon plot kinetic analyses and Molecular docking, respectively. The \u03b1\u2010amylase inhibition assay revealed noncompetitive inhibition for both compounds with IC<jats:sub>50<\/jats:sub> and Ki values of 4.74\u00b10.17 and 4.75\u2005mM for compound <jats:bold>1<\/jats:bold>, and 11.29\u00b10.44 and 12.37\u2005mM for compound <jats:bold>2<\/jats:bold>, respectively. In comparison, the standard drug acarbose displayed a competitive mode of inhibition, with IC<jats:sub>50<\/jats:sub> and Ki values of 11.99\u00b10.02 and 12.68\u2005mM, respectively. The binding affinities with \u03b1\u2010amylase were \u22126.42 and \u22126.07\u2005kcal\/mol for compounds <jats:bold>1<\/jats:bold> and <jats:bold>2<\/jats:bold>, respectively relative to acarbose \u22128.03\u2005Kcal\/mol. Moreover, these two compounds\u2019 predicted physicochemical and ADMET properties justified their potential as lead compounds for drug discovery. These compounds demonstrated remarkable inhibition potential comparable to the standard drug, highlighting their potential as viable alternatives in managing diabetes.<\/jats:p>","DOI":"10.1002\/cbdv.202401515","type":"journal-article","created":{"date-parts":[[2024,11,4]],"date-time":"2024-11-04T17:13:48Z","timestamp":1730740428000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Alkaloids Isolated from <i>Vepris glandulosa<\/i> with Antidiabetic Properties: An <i>In Vitro<\/i> and <i>In Silico<\/i> Analysis"],"prefix":"10.1002","volume":"22","author":[{"given":"Prince","family":"Ojuka","sequence":"first","affiliation":[{"name":"Department of Physical Science University of Embu  P.O Box 6-60100 Embu Kenya"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles O.","family":"Ochieng","sequence":"additional","affiliation":[{"name":"Department of Chemistry Maseno University  Private Bag, Maseno Kenya"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wilberforce","family":"Ndarawit","sequence":"additional","affiliation":[{"name":"Department of Physical Science University of Embu  P.O Box 6-60100 Embu Kenya"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel W.","family":"Nyongesa","sequence":"additional","affiliation":[{"name":"Department of Chemistry Maseno University  Private Bag, Maseno Kenya"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Justus","family":"Mukavi","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Biology and Phytochemistry (IPBP) University of M\u00fcnster  PharmaCampus Corrensstrasse 48 Muenster D-48149 Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James","family":"Nyabuga Nyariki","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biotechnology Technical of University of Kenya  P.O Box 52428-00200 Nairobi Kenya"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seth","family":"Apollo","sequence":"additional","affiliation":[{"name":"Department of Physical Science University of Embu  P.O Box 6-60100 Embu Kenya"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cleydson B. 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