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It was found that most of the synthesized imidazolopyrazin derivatives exhibited excellent inhibitory effects towards three tested tutor cells <jats:italic>in\u2009vitro<\/jats:italic>. Among them, three compounds have IC<jats:sub>50<\/jats:sub> values much lower than Fluorouracil while show low toxicity to normal cells L\u201002. The migration ability assay have proved that <jats:bold>A6<\/jats:bold> and <jats:bold>A9<\/jats:bold> effectively inhibit the migration of tumor cells. Reverse molecular docking studies indicated that the potent targets of these derivatives are JAKs as they well docked into kinases with low energy. These finding suggest that imidazo[1,5\u2010a]pyrazin derivatives may be lead compounds for developing potent JAK targeted anticancer candidates.<\/jats:p>","DOI":"10.1002\/cbdv.202401553","type":"journal-article","created":{"date-parts":[[2024,11,8]],"date-time":"2024-11-08T13:07:28Z","timestamp":1731071248000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Design, Synthesis, Anticancer Evaluation and <i>In Silico<\/i> Studies of Imidazole Pyrazine Compounds"],"prefix":"10.1002","volume":"22","author":[{"given":"Gong","family":"Chen","sequence":"first","affiliation":[{"name":"School of Pharmacy Guangdong Pharmaceutical University  Guangzhou 510006 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiwei","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Guiyang Maternity and Child Health Hospital  Guiyang 550003 People's 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