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Its remarkable efficacy in treating immune\u2010related and inflammatory disorders has garnered significant interest among scientific circles. However, the anti\u2010inflammatory and antioxidant effects of ACT and its underlying molecular mechanisms require further investigation. In this study, pharmacophore\u2010based reverse docking and molecular dynamics simulations identified potential anti\u2010inflammatory targets in silico. Studies conducted in\u2005vitro with lipopolysaccharide (LPS)\u2010induced RAW264.7 cells validated the anti\u2010inflammatory properties of ACT. Methyl thiazolyl tetrazolium (MTT) and lactate dehydrogenase (LDH) assays indicated ACT's non\u2010toxic and growth\u2010promoting effects on cells. ACT significantly reduced nitric oxide (NO) and reactive oxygen species (ROS) production and restored levels of antioxidant enzymes. It also decreased pro\u2010inflammatory cytokines. Western blotting assays indicated that ACT inhibited p38, TNF\u2010\u03b1, PI3\u2005K\/AKT, and NF\u2010\u03baB signaling pathways. These findings underscore ACT's ability to mitigate acute inflammation in RAW264.7 cells by modulating key signaling pathways and provide the scientific basis for enhancing the medicinal value of ACT and future drug development.<\/jats:p>","DOI":"10.1002\/cbdv.202401564","type":"journal-article","created":{"date-parts":[[2024,10,4]],"date-time":"2024-10-04T12:33:40Z","timestamp":1728045220000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Unraveling the Dual Anti\u2010Inflammatory and Antioxidant Mechanisms of Acteoside: Computational Insights and Experimental Validation"],"prefix":"10.1002","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7583-9677","authenticated-orcid":false,"given":"Eldar","family":"Muhtar","sequence":"first","affiliation":[{"name":"Institute of Agro-products Storage and Processing, Xinjiang Key Laboratory of Processing and Preservation of Agricultural Products Xinjiang Academy of Agricultural Science  Urumqi, Xinjiang 830091 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gulfira","family":"Ylham","sequence":"additional","affiliation":[{"name":"Institute of Agro-products Storage and Processing, Xinjiang Key Laboratory of Processing and Preservation of Agricultural Products Xinjiang Academy of Agricultural Science  Urumqi, Xinjiang 830091 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atawulla","family":"Tiemuer","sequence":"additional","affiliation":[{"name":"Institute of Agro-products Storage and Processing, Xinjiang Key Laboratory of Processing and Preservation of Agricultural Products Xinjiang Academy of Agricultural Science  Urumqi, Xinjiang 830091 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-1899-2372","authenticated-orcid":false,"given":"Salamet","family":"Edirs","sequence":"additional","affiliation":[{"name":"Institute of Agro-products Storage and Processing, Xinjiang Key Laboratory of Processing and Preservation of Agricultural Products Xinjiang Academy of Agricultural Science  Urumqi, Xinjiang 830091 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,11,7]]},"reference":[{"key":"e_1_2_11_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2021.114493"},{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2020.110452"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.870239"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2018.11.003"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2023.e21032"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/medicines5030106"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/7008907"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/biof.1831"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24010095"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2021.113991"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/med.21768"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/2731289"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2015.09.026"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2015.03.020"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2021.174699"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2024.116607"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2023.109496"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2023.109806"},{"issue":"6","key":"e_1_2_11_19_1","first-page":"25","volume":"37","author":"Niu Y. 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