{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T01:49:07Z","timestamp":1773280147266,"version":"3.50.1"},"reference-count":27,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,2,14]],"date-time":"2025-02-14T00:00:00Z","timestamp":1739491200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,2,14]],"date-time":"2025-02-14T00:00:00Z","timestamp":1739491200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Malaria remains a significant public health challenge in Niger, accounting for 5.6% of global malaria\u2010related deaths. Local medicinal plants are frequently used as traditional treatments for malaria, although their efficacy and safety are often insufficiently investigated. This study aims to evaluate the antiplasmodial activity and chemical composition of eight medicinal plants from Niger. Plant extracts were tested in vitro for their ability to inhibit the uptake of [\n                    <jats:sup>3<\/jats:sup>\n                    H]\u2010hypoxanthine in the\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    NF54 chloroquine\u2010sensitive strain. The most active extract was subjected to chemical analysis using HPLC\u2013PDA\u2013HRMS\/MS dereplication, whereas major compounds were quantified via a validated LC\u2013UV method. The ethyl acetate extract of\n                    <jats:italic>Combretum aculeatum<\/jats:italic>\n                    (\n                    <jats:bold>2a<\/jats:bold>\n                    ) demonstrated important antiplasmodial activity, with an IC\n                    <jats:sub>50<\/jats:sub>\n                    value of 3.1\u00a0\u00b5g\/mL and a selectivity index of 24.5.\n                    <jats:italic>C<\/jats:italic>\n                    \u2010glycosyl flavonoids were identified as the primary constituents, present at concentrations ranging from 5.7 to 9.8\u00a0mg of vitexin equivalent per g of extract. However, the low abundance of these compounds suggests that other constituents may contribute to the extract's antiplasmodial effects. Further investigations are required to explore possible synergies among the components of\n                    <jats:bold>2a<\/jats:bold>\n                    and to assess its efficacy in vivo models.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202401621","type":"journal-article","created":{"date-parts":[[2025,1,30]],"date-time":"2025-01-30T06:39:51Z","timestamp":1738219191000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["In Vitro Antiplasmodial Activity and Chemical Composition of\n                    <i>Combretum aculeatum<\/i>\n                    , a Medicinal Plant From Niger"],"prefix":"10.1002","volume":"22","author":[{"given":"Souleymane Magagi","family":"Dam\u00e9","sequence":"first","affiliation":[{"name":"Faculty of Sciences and Techniques Natural Products Laboratory, Abdou Moumouni University  Niamey Niger"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Louis","family":"Leclercq","sequence":"additional","affiliation":[{"name":"UMR 7200 Therapeutic Innovation Laboratory Strasbourg University, CNRS, Strasbourg Institute for Drug Discovery and Development (IMS)  Strasbourg France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Vonthron\u2010S\u00e9n\u00e9cheau","sequence":"additional","affiliation":[{"name":"UMR 7200 Therapeutic Innovation Laboratory Strasbourg University, CNRS, Strasbourg Institute for Drug Discovery and Development (IMS)  Strasbourg France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amadou Tidjani","family":"Ilagouma","sequence":"additional","affiliation":[{"name":"Faculty of Sciences and Techniques Natural Products Laboratory, Abdou Moumouni University  Niamey Niger"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1596-4887","authenticated-orcid":false,"given":"Sergio","family":"Ortiz","sequence":"additional","affiliation":[{"name":"UMR 7200 Therapeutic Innovation Laboratory Strasbourg University, CNRS, Strasbourg Institute for Drug Discovery and Development (IMS)  Strasbourg France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,2,14]]},"reference":[{"key":"e_1_2_9_2_1","volume-title":"Global Malaria Program (GMP), World Malaria Report 2023","author":"W. H. O. Team","year":"2023"},{"key":"e_1_2_9_3_1","volume-title":"WHO Traditional Medicine Strategy: 2014\u20102023","author":"W. H. O. Team","year":"2013"},{"key":"e_1_2_9_4_1","first-page":"812","article-title":"Diversity of Healing Plants Used in the Traditional Treatment of Malaria in Four Municipalities of Niger","volume":"5","author":"Soumaila M.","year":"2017","journal-title":"Asian Journal of Applied Sciences"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2023\/8296195"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phyplu.2024.100615"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.19044\/esj.2017.v13n33p276"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1211\/jpp\/61.11.0001"},{"key":"e_1_2_9_9_1","first-page":"303","article-title":"Anti\u2010Plasmodial Activity of Chloroform Leaf Extract of Eucalyptus Camaldulensis in Mice","volume":"22","author":"Ishaya Y.","year":"2019","journal-title":"African Journal of Biomedical Research"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3746\/pnf.2020.25.1.58"},{"key":"e_1_2_9_11_1","first-page":"302","article-title":"The Antimalarial Potential of Medicinal Plants Used for the Treatment of Malaria in Cameroonian Folk Medicine","volume":"5","author":"Titanji V.","year":"2008","journal-title":"African Journal of Traditional, Complementary and Alternative Medicines"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2010.10.039"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2010.04.032"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.4314\/ajtcam.v3i4.31175"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jms.3803"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jms.995"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2019.112052"},{"key":"e_1_2_9_18_1","volume-title":"Committee for Medicinal Products for Human Use (CHMP) Guideline on Bioanalytical Method Validation","author":"EMA","year":"2009"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.4314\/ajid.v3i1.55070"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2023.117394"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12936\u2010016\u20101278\u20106"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2020.113624"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1080\/13880200701214821"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436\u2010011\u20102519\u20109"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978\u20100\u201012\u2010442704\u20104.50021\u20109"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0001\u2010706X(97)00079\u2010X"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550\u20107408.1977.tb00987.x"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022\u20101759(94)90396\u20104"}],"container-title":["Chemistry &amp; Biodiversity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.202401621","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbdv.202401621","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.202401621","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T11:07:01Z","timestamp":1773227221000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbdv.202401621"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,14]]},"references-count":27,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,6]]}},"alternative-id":["10.1002\/cbdv.202401621"],"URL":"https:\/\/doi.org\/10.1002\/cbdv.202401621","archive":["Portico"],"relation":{},"ISSN":["1612-1872","1612-1880"],"issn-type":[{"value":"1612-1872","type":"print"},{"value":"1612-1880","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,14]]},"assertion":[{"value":"2024-07-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e202401621"}}