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This study investigated the antioxidant and antiproliferative effects of methanolic fruit extract of <jats:italic>S. xanthocarpum<\/jats:italic> on human breast cancer cells (MDA\u2010MB\u2010231). Phytochemical screening and LC\u2010HRMS analysis confirmed presence of various primary and secondary metabolites. Antioxidant activity was assessed through DPPH, ABTS radical scavenging, reducing power, and phosphomolybdate assays. The extract demonstrated significant antioxidant potential with EC<jats:sub>50<\/jats:sub> values of 60.10\u00b10.88\u2005\u03bcg\/mL (DPPH) and 392.29\u00b13.93\u2005\u03bcg\/mL (ABTS). Cytotoxicity against MDA\u2010MB\u2010231 cells was evaluated <jats:italic>via<\/jats:italic> morphological analysis, MTT assays, and IC<jats:sub>50<\/jats:sub> determination (24.19\u00b10.56\u2005\u03bcg\/L). Apoptosis was confirmed using dual staining techniques (AO\/EB, Hoechst 33342\/PI, DAPI), revealing condensed nuclei, apoptotic bodies, and reduced mitochondrial membrane potential, as indicated by Rhodamine staining. Additionally, increased reactive oxygen species (ROS) levels were observed using H2\u2010DCF\u2010DA staining. The total phenolic and flavonoid contents of the extract were 127.78\u00b13.547\u2005mg\u2009GAE\/g and 98.06\u00b14.289\u2005mg\u2009QE\/g, respectively. These findings suggest that the methanolic fruit extract of <jats:italic>S. xanthocarpum<\/jats:italic> possesses strong antioxidant and anticancer activities, indicating its potential role in cancer treatment. Further studies are warranted to explore its bioactive compounds for developing novel anticancer therapies.<\/jats:p>","DOI":"10.1002\/cbdv.202401672","type":"journal-article","created":{"date-parts":[[2024,10,4]],"date-time":"2024-10-04T07:04:35Z","timestamp":1728025475000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Antiproliferative Effects of Methanolic Fruit Extract of <i>Solanum xenthocarpum<\/i> (L.) on Human Breast Cancer Cells"],"prefix":"10.1002","volume":"22","author":[{"given":"Saumya","family":"Maurya","sequence":"first","affiliation":[{"name":"Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Rajiv Gandhi South Campus Banaras Hindu University  Mirzapur 231001 Uttar Pradesh, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kumari","family":"Swati","sequence":"additional","affiliation":[{"name":"Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Rajiv Gandhi South Campus Banaras Hindu University  Mirzapur 231001 Uttar Pradesh, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Suvetha","sequence":"additional","affiliation":[{"name":"Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Rajiv Gandhi South Campus Banaras Hindu University  Mirzapur 231001 Uttar Pradesh, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mayukh","family":"Ghosh","sequence":"additional","affiliation":[{"name":"Department of Veterinary Physiology &amp; Biochemistry, Faculty of Veterinary and Animal Sciences, I.Ag.Scs., Rajiv Gandhi South Campus Banaras Hindu University  Mirzapur 231001 Uttar Pradesh, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1847-7486","authenticated-orcid":false,"given":"Pavan Kumar","family":"Yadav","sequence":"additional","affiliation":[{"name":"Department of Veterinary Physiology &amp; Biochemistry, Faculty of Veterinary and Animal Sciences, I.Ag.Scs., Rajiv Gandhi South Campus Banaras Hindu University  Mirzapur 231001 Uttar Pradesh, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,11,12]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"crossref","unstructured":"K.Mudie MM.Jin Tan L. 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