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The latter bacteria are emerging microorganisms that pose significant challenges due to their resistance to a broad range of potent therapeutic drugs.\n                  <\/jats:p>\n                  <jats:p>\n                    The extract was prepared through an accessible acid hydrolysis method.\n                    <jats:italic>In vitro<\/jats:italic>\n                    and\n                    <jats:italic>In silico<\/jats:italic>\n                    analyses through MIC, MBC analysis and molecular docking were conducted to evaluate the antibacterial properties.\n                  <\/jats:p>\n                  <jats:p>\n                    The extract showed remarkable antioxidant activity and significant antibacterial potential against reference species and ESBL bacteria. MIC and MBC calculations confirmed the extract's capacity to kill bacteria rather than just inhibit their growth. Further\n                    <jats:italic>in silico<\/jats:italic>\n                    analyzes demonstrated the high binding affinity of HT to the active sites of the gyrase B subunit and the peptidoglycan DD\u2010transpeptidase domain from proteins located in the cytoplasm and the cell wall of the bacteria, respectively. Results confirmed the structure\u2010activity relationship and the ability of HT to disrupt essential bacterial functions.\n                  <\/jats:p>\n                  <jats:p>This study validates the debated antimicrobial potential of HT and highlights its importance as a potential therapeutic agent against resistant bacteria, which is a critical area of research given the global challenge of antibiotic resistance.<\/jats:p>","DOI":"10.1002\/cbdv.202401714","type":"journal-article","created":{"date-parts":[[2024,9,18]],"date-time":"2024-09-18T22:23:36Z","timestamp":1726698216000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["New Insights into the Antibacterial Activity of Hydroxytyrosol Extracted from Olive Leaves: Molecular Docking Simulations of its Antibacterial Mechanisms"],"prefix":"10.1002","volume":"22","author":[{"given":"Amal","family":"Ben Hassena","sequence":"first","affiliation":[{"name":"Laboratory of Electrochemistry and Environment National School of Engineers of Sfax University of Sfax, BP 1173  Sfax 3038 Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jihen","family":"Abidi","sequence":"additional","affiliation":[{"name":"Laboratory of Electrochemistry and Environment National School of Engineers of Sfax University of Sfax, BP 1173  Sfax 3038 Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nabil","family":"Miled","sequence":"additional","affiliation":[{"name":"College of Science Department of Biological Sciences University of Jeddah  Jeddah Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u0141ukasz","family":"Kulinowski","sequence":"additional","affiliation":[{"name":"Department of Natural Products Chemistry Medical University of Lublin  1 Chodzki Street 20-093 Lublin Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krystyna","family":"Skalicka\u2010Wo\u017aniak","sequence":"additional","affiliation":[{"name":"Department of Natural Products Chemistry Medical University of Lublin  1 Chodzki Street 20-093 Lublin Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9107-7027","authenticated-orcid":false,"given":"Mohamed","family":"Bouaziz","sequence":"additional","affiliation":[{"name":"Laboratory of Electrochemistry and Environment National School of Engineers of Sfax University of Sfax, BP 1173  Sfax 3038 Tunisia"},{"name":"Higher Institute of Biotechnology of Sfax University of Sfax, BP 1175  Sfax 3038 Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,31]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajic.2011.05.020"},{"key":"e_1_2_8_2_1","doi-asserted-by":"crossref","unstructured":"B. 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