{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,4]],"date-time":"2025-09-04T13:12:24Z","timestamp":1756991544112,"version":"3.41.2"},"reference-count":36,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,12,7]],"date-time":"2024-12-07T00:00:00Z","timestamp":1733529600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2022A1515010783"],"award-info":[{"award-number":["2022A1515010783"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Screening A\u03b2 aggregation inhibitors (AAIs) is important for Alzheimer's disease drug discovery. However, common cellular or biochemical methods are not suitable for high\u2010throughput natural product screening. A gold nanoparticle (GNP) screening method was employed in this study to screen marine fungal crude extracts and pure compounds for quick AAI discovering. The anti\u2010A\u03b2 aggregation activity was further inspected using transmission electron microscopic (TEM) observation, and the interaction between active molecules and different A\u03b2 species was revealed by molecular docking. The results indicated that the fungal extracts DLS2008001(M), BM3T2(M), DLEN2008005(M), TBG1\u201016(P), and TBG1\u201013(P) showed activity comparable to the positive control human serum albumin at the concentration of 500\u00a0\u00b5g\/mL; 10 pure compounds also displayed moderate anti\u2010aggregation activity, particularly nidulin, aspergillusidone G, and butyrolactone I. The inspection of anti\u2010A\u03b2 aggregation effect through TEM further demonstrated that extracts TBG1\u201016(P), DLS2008001(M), and BM3T2(M) dramatically inhibited the formation of A\u03b2 aggregates. Molecular docking displayed low binding energies and key interactions of nidulin, aspergillusidone G, and butyrolactone I, with nine types of A\u03b2 peptides. These findings indicate that the GNP method is efficient in screening AAIs and reveal marine fungal natural products as valuable sources of small molecular AAIs.<\/jats:p>","DOI":"10.1002\/cbdv.202401809","type":"journal-article","created":{"date-parts":[[2024,11,27]],"date-time":"2024-11-27T08:28:57Z","timestamp":1732696137000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Screening for Anti\u2010A\u03b2 Aggregation Activity of Marine Fungal Natural Products Based on a Gold Nanoparticle Method"],"prefix":"10.1002","volume":"22","author":[{"given":"Xingyuan","family":"Wang","sequence":"first","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety Guangdong Provincial Engineering Laboratory for Marine Biological Products Zhanjiang Municipal Key Laboratory of Marine Drugs and Nutrition for Brain Health Research Institute for Marine Drugs and Nutrition College of Food Science and Technology Marine Biomedicine R&amp;D Center at Shenzhen Institute of Guangdong Ocean University Guangdong Ocean University Zhanjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Longjian","family":"Zhou","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety Guangdong Provincial Engineering Laboratory for Marine Biological Products Zhanjiang Municipal Key Laboratory of Marine Drugs and Nutrition for Brain Health Research Institute for Marine Drugs and Nutrition College of Food Science and Technology Marine Biomedicine R&amp;D Center at Shenzhen Institute of Guangdong Ocean University Guangdong Ocean University Zhanjiang China"},{"name":"Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang) Zhanjiang China"},{"name":"Collaborative Innovation Center of Seafood Deep Processing Dalian Polytechnic University Dalian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yayue","family":"Liu","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety Guangdong Provincial Engineering Laboratory for Marine Biological Products Zhanjiang Municipal Key Laboratory of Marine Drugs and Nutrition for Brain Health Research Institute for Marine Drugs and Nutrition College of Food Science and Technology Marine Biomedicine R&amp;D Center at Shenzhen Institute of Guangdong Ocean University Guangdong Ocean University Zhanjiang China"},{"name":"Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang) Zhanjiang China"},{"name":"Collaborative Innovation Center of Seafood Deep Processing Dalian Polytechnic University Dalian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fangfang","family":"Ban","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety Guangdong Provincial Engineering Laboratory for Marine Biological Products Zhanjiang Municipal Key Laboratory of Marine Drugs and Nutrition for Brain Health Research Institute for Marine Drugs and Nutrition College of Food Science and Technology Marine Biomedicine R&amp;D Center at Shenzhen Institute of Guangdong Ocean University Guangdong Ocean University Zhanjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiyou","family":"Yang","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety Guangdong Provincial Engineering Laboratory for Marine Biological Products Zhanjiang Municipal Key Laboratory of Marine Drugs and Nutrition for Brain Health Research Institute for Marine Drugs and Nutrition College of Food Science and Technology Marine Biomedicine R&amp;D Center at Shenzhen Institute of Guangdong Ocean University Guangdong Ocean University Zhanjiang 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