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This study aimed to evaluate the chemical composition, antiradical and antioxidant activities, enzyme inhibitory activities, and anti\u2010inflammatory properties of various extracts from the aerial parts of <jats:italic>E. spectabilis<\/jats:italic>. Various assays were used to investigate the antioxidant and enzyme inhibitory properties. The chemical composition of the tested extracts was analyzed using High\u2010Performance Liquid Chromatography\u2010Electrospray Ionization Tandem Mass Spectrometry (HPLC\u2010ESI\u2010MS\/MS). Additionally, the extracts were tested on isolated mouse colon tissue challenged with <jats:italic>E. coli<\/jats:italic> lipopolysaccharide (LPS) to replicate the inflammation and oxidative stress burden characteristic of inflammatory bowel diseases. In the chemical composition, vanillic, ferulic, and 4\u2010hydroxybenzoic acids were the prominent compounds. The greatest antioxidant activity was observed in the methanol and water extracts from the aerial parts. Enzyme inhibition tests showed that the ethyl acetate extract had the highest anti\u2010acetylcholinesterase activity. The gene expression of pro\u2010inflammatory cyclooxygenase\u20102 (COX\u20102) and pro\u2010oxidant inducible nitric oxide synthase (iNOS) biomarkers were assayed. Among the extracts, the methanol extract was the most effective in blunting LPS\u2010induced gene expression of COX\u20102. <jats:italic>E. spectabilis<\/jats:italic> may serve as a valuable source of phytochemicals for combating oxidative stress and inflammation\u2010driven diseases, with a particular emphasis on colon inflammatory condition.<\/jats:p>","DOI":"10.1002\/cbdv.202401881","type":"journal-article","created":{"date-parts":[[2024,10,22]],"date-time":"2024-10-22T11:19:06Z","timestamp":1729595946000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Investigation of the Chemical Composition and Biological Activities of <i>Eremurus spectabilis<\/i> M. 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