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The present study was conucted to elucidate the effects and molecular mechanisms of <jats:italic>Tremella fuciformis<\/jats:italic>\u2010derived polysaccharides (PTP\u20103a) on glucose and lipid metabolism in palmitic acid (PA) \u2010 treated HepG2 cells. Multiple parameters were assessed following PTP\u20103a treatment, including lipid accumulation, glycogen content, glucose consumption, and enzyme activities, including pyruvate kinase (PK) and hexokinase (HK). Additionally, the expression levels of genes associated with glucose and lipid metabolism was evaluated using western blot analysis. PTP\u20103a effectively inhibited lipid accumulation, promoted the glucose consumption, increased the amount of cellular glycogen, and enhanced PK and HK activities in PA\u2010treated cells. Furthermore, PTP\u20103a induced a significant increase in the p\u2010AMPK\/AMPK ratio and the expression level of PPARa, while decreasing the expression levels of SREBP, FAS, ACC, and SOCS3. In conclusion, these findings suggested that PTP\u20103a exerted beneficial effects on glucose and lipid metabolism by activating the AMPK signaling pathway, resulting in the inhibition of lipogenesis, promotion of fatty acid oxidation, and enhancement of cellular glycogen synthesis and glycolysis. These findings hold clinical relevance and provide a foundation for potential treatments for non\u2010alcoholic fatty liver disease (NAFLD) and and related metabolic disorders.<\/jats:p>","DOI":"10.1002\/cbdv.202401926","type":"journal-article","created":{"date-parts":[[2024,9,25]],"date-time":"2024-09-25T09:45:52Z","timestamp":1727257552000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Polysaccharides from <i>Tremella Fuciformis<\/i> Enhance Glucose and Lipid Metabolism in HepG2 Cells Through Activating the AMPK Signaling Pathway"],"prefix":"10.1002","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1091-3084","authenticated-orcid":false,"given":"Haizhao","family":"Song","sequence":"first","affiliation":[{"name":"College of Food Science and Engineering Nanjing University of Finance and Economics \/Collaborative Innovation Center for Modern Grain Circulation and Safety  Nanjing 210023 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Lu","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering Nanjing University of Finance and Economics \/Collaborative Innovation Center for Modern Grain Circulation and Safety  Nanjing 210023 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rou","family":"Deng","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering Nanjing University of Finance and Economics \/Collaborative Innovation Center for Modern Grain Circulation and Safety  Nanjing 210023 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,11,8]]},"reference":[{"key":"e_1_2_10_1_1","doi-asserted-by":"publisher","DOI":"10.1007\/s42000-018-0014-8"},{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42255-019-0145-5"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cld.2015.10.001"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1204265"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-018-0104-9"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.5402\/2011\/363546"},{"key":"e_1_2_10_7_1","doi-asserted-by":"crossref","unstructured":"L. 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