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Among them, ECA (<jats:bold>5<\/jats:bold>) showed potential anti\u2010osteoclastogenic activity. To the best of our knowledge, this represents the first isolation of ECA (<jats:bold>5<\/jats:bold>) from <jats:italic>P. chinensis<\/jats:italic> as well as the first investigation of its effects on osteoclast formation. Further study revealed that ECA inhibited RANKL\u2010induced mature osteoclast formation <jats:italic>in\u2005vitro<\/jats:italic> without compromising cell viability. Mechanistically, ECA attenuated RANKL\u2010induced mitogen\u2010activated protein kinases (MAPKs) and nuclear factor\u2010\u03baB (NF\u2010\u03baB) activation, leading to the inhibition of c\u2010Fos and nuclear factor of activated T cells cytoplasmic 1 (NFATc1) activation. Moreover, ECA protected against LPS\u2010induced inflammatory bone loss and osteoclast formation in a mouse model. However, ECA did not inhibit LPS\u2010induced inflammatory responses in macrophages. Our findings suggest that ECA mitigates LPS\u2010induced inflammatory bone loss in mice by inhibiting RANKL\u2010induced activation of key osteoclastogenic transcription factors, including c\u2010Fos and NFATc1, and may be a potential natural triterpenoid for preventing or treating osteolytic diseases.<\/jats:p>","DOI":"10.1002\/cbdv.202402011","type":"journal-article","created":{"date-parts":[[2024,11,14]],"date-time":"2024-11-14T06:49:48Z","timestamp":1731566988000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Triterpenoids from <i>Potentilla chinensis<\/i> Inhibit RANKL\u2010Induced Osteoclastogenesis <i>in Vitro<\/i> and Lipopolysaccharide\u2010Induced Osteolytic Bone Loss <i>in Vivo<\/i>"],"prefix":"10.1002","volume":"22","author":[{"given":"Trong Trieu","family":"Tran","sequence":"first","affiliation":[{"name":"College of Pharmacy, Drug Research and Development Center Daegu Catholic University  Gyeongbuk 38430 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minju","family":"Gal","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, College of Natural Sciences Kangwon National University  Chuncheon, Gangwon-Do 24341 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manh Tuan","family":"Ha","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Drug Research and Development Center Daegu Catholic University  Gyeongbuk 38430 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seungeun","family":"Hyun","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, College of Natural Sciences Kangwon National University  Chuncheon, Gangwon-Do 24341 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Okwha","family":"Kim","sequence":"additional","affiliation":[{"name":"Kangwon Institute of Inclusive Technology Kangwon National University  Chuncheon, Gangwon-Do 24341 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeong\u2010Hyung","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, College of Natural Sciences Kangwon National University  Chuncheon, Gangwon-Do 24341 Republic of Korea"},{"name":"Kangwon Institute of Inclusive Technology Kangwon National University  Chuncheon, Gangwon-Do 24341 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8501-4289","authenticated-orcid":false,"given":"Byung Sun","family":"Min","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Drug Research and Development Center Daegu Catholic University  Gyeongbuk 38430 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,11,29]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hermed.2021.100460"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2008.12.022"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2021.104659"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-17393-8"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2016.5679"},{"key":"e_1_2_8_6_1","first-page":"237","volume":"29","author":"Shen Y.","year":"2006","journal-title":"J. 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