{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T01:56:13Z","timestamp":1773280573668,"version":"3.50.1"},"reference-count":53,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,3,13]],"date-time":"2025-03-13T00:00:00Z","timestamp":1741824000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,3,13]],"date-time":"2025-03-13T00:00:00Z","timestamp":1741824000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Antibiotic resistance is one of the biggest threats to global health. Fungal endophytes are important sources of active natural products with antimicrobial potential. The purpose of this study was to characterize the endophytes coexisting with\n                    <jats:italic>Helichrysum oocephalum<\/jats:italic>\n                    , evaluate their antimicrobial activities, and annotate the endophytes metabolites. Six fungal species, including\n                    <jats:italic>Fusarium avenaceum<\/jats:italic>\n                    and\n                    <jats:italic>Fusarium tricinctum<\/jats:italic>\n                    , were identified. Endophytes were cultured, and their metabolites were extracted. The antimicrobial effects of the extracts were tested against\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    ,\n                    <jats:italic>Bacillus cereus, Staphylococcus epidermidis<\/jats:italic>\n                    ,\n                    <jats:italic>Pseudomonas aeruginosa, Escherichia coli<\/jats:italic>\n                    , and\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    . In addition, anti\u2010biofilm effects of the extracts were examined against\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    and\n                    <jats:italic>S. epidermidis<\/jats:italic>\n                    . The metabolites in the most active extract were annotated on the basis of the LC\u2010ESI\u2010QToF\u2010MS\/MS data. In anti\u2010biofilm studies,\n                    <jats:italic>F. avenaceum<\/jats:italic>\n                    extract was effective in destroying and inhibiting the biofilm formation of\n                    <jats:italic>S. epidermidis<\/jats:italic>\n                    . LC\u2013MS analysis showed that most of the identified compounds in the active extracts were enniatins (cyclic hexadepsipeptides). However, apicidin derivatives were also annotated. Our results revealed that these endophytes, especially\n                    <jats:italic>Fusarium<\/jats:italic>\n                    species, have antimicrobial activity against\n                    <jats:italic>S. aureus<\/jats:italic>\n                    ,\n                    <jats:italic>B. cereus<\/jats:italic>\n                    , and\n                    <jats:italic>C. albicans<\/jats:italic>\n                    and anti\u2010biofilm activity against\n                    <jats:italic>S. epidermidis<\/jats:italic>\n                    . According to the literature, the observed antimicrobial activity can be attributed to the enniatins. However, further phytochemical and pharmacological studies are necessary in this regard.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202402236","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T12:09:00Z","timestamp":1741694940000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Isolation and Characterization of Fungal Endophytes From\n                    <i>Helichrysum oocephalum<\/i>\n                    , Evaluating Their Antimicrobial Activities, and Annotation of Their Metabolites"],"prefix":"10.1002","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-8215-8192","authenticated-orcid":false,"given":"Reza","family":"Mostafazade","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy School of Pharmacy Mashhad University of Medical Sciences Mashhad Iran"},{"name":"Biotechnology Research Center Pharmaceutical Technology Institute Mashhad University of Medical Sciences Mashhad Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7324-3973","authenticated-orcid":false,"given":"Zahra","family":"Tazik","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy School of Pharmacy Mashhad University of Medical Sciences Mashhad Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4298-3132","authenticated-orcid":false,"given":"Seyed Ahmad","family":"Emami","sequence":"additional","affiliation":[{"name":"Department of Traditional Pharmacy School of Pharmacy Mashhad University of Medical Sciences Mashhad Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5460-5273","authenticated-orcid":false,"given":"Karel","family":"Nesm\u011br\u00e1k","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry Faculty of Science Charles University Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8008-7668","authenticated-orcid":false,"given":"Martin","family":"\u0160t\u00edcha","sequence":"additional","affiliation":[{"name":"Mass Spectrometry Laboratory Section of Chemistry Faculty of Science Charles University Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5047-797X","authenticated-orcid":false,"given":"Vahid","family":"Soheili","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Control School of Pharmacy Mashhad University of Medical Sciences Mashhad Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3971-2377","authenticated-orcid":false,"given":"Maryam","family":"Akaberi","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy School of Pharmacy Mashhad University of Medical Sciences Mashhad Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,3,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573-020-00114-z"},{"key":"e_1_2_9_3_1","first-page":"153","article-title":"Bioactive Natural Products From Endophytes: A Review","volume":"44","author":"Guo B.","year":"2008","journal-title":"Prikladnaia Biokhimiia I Mikrobiologiia"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1039\/b609472b"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcab.2024.103307"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2016.10.097"},{"key":"e_1_2_9_7_1","volume-title":"Plants of the World Online","author":"POWO","year":"2025"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jnatprod.8b01031"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2021.114196"},{"key":"e_1_2_9_10_1","first-page":"1045","article-title":"Hypericum perforatum: Traditional Uses, Clinical Trials, and Drug Interactions","volume":"25","author":"Nobakht S. 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