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Traditionally, various part of <jats:italic>H. aromatica<\/jats:italic> such as leaves and rhizomes were applied to treat joint pain, skin diseases, colds, asthma, diarrhea, and jaundice. Whole herbs extracts are used to address muscle weakness, rheumatism, and allergies, purify the blood, treat deafness, and promote wound healing, especially in postpartum women. The present study is subjected to assess anti\u2010inflammatory, anti\u2010diabetic, tyrosinase inhibitory, acetylcholinesterase (AChE), antimicrobial, and genotoxic efficacies of <jats:italic>H. aromatica<\/jats:italic> rhizome essential oil (HAEO). To validate the findings of biological assays, in silico methods including Prediction of Activity Spectra for Substances (PASS) tool, target prediction, and molecular docking were employed. Using Clevenger apparatus dry rhizome of HAEO was extracted. The chemical profiling was performed using gas chromatography\/mass spectrometry (GC\/MS) where HAEO yield was 1.09%\u00a0\u00b1\u00a00.641% (v\/w) with 12 compounds where linalool was identified as major compound (76.29%). Anti\u2010inflammatory activity showed IC<jats:sub>50<\/jats:sub> value for protease inhibitory assay 19.59\u00a0\u00b5L\/mL and albumin denaturation assay 32.16\u00a0\u00b5L\/mL. The IC<jats:sub>50<\/jats:sub> value for \u03b1\u2010amylase inhibitory activity showed 29.84\u00a0\u00b5L\/mL, whereas tyrosinase inhibitory activity showed 73.62\u00a0\u00b5L\/mL. Acetylcholinesterase inhibitory (AChEase) activity revealed an IC<jats:sub>50<\/jats:sub> value of 38.13\u00a0\u00b5L\/mL. Antimicrobial activity showed minimal inhibitory concentration (MIC) values of 10\u00a0\u00b1\u00a00.47 and 45\u00a0\u00b1\u00a00.47\u00a0\u00b5g\/mL against <jats:italic>Staphylococcus aureus<\/jats:italic> and <jats:italic>Candida albicans<\/jats:italic>. Genotoxicity analysis showed HAEO has moderate toxic effect. In silico results identified six potential targets via Swiss target prediction: (AChE, CYP51, peroxisome proliferator\u2010activated receptor [PPAR], cyclooxygenase\u20102 [COX\u20102], and tyrosinase\u2010related protein 1 [TYRP1]). Additionally, molecular docking studies revealed Linalool \u22127.4\u00a0kcal\/mol, Spathulenol \u22128.5\u00a0kcal\/mol, aromadendrene oxide\u2010(2) \u22128.3\u00a0kcal\/mol better docking scores. For the first time, this study reports on anti\u2010diabetic, anti\u2010tyrosinase, acetylcholinesterase inhibitory activities and genotoxicity of HAEO. In silico investigation supports these activities by evaluating binding affinities and interactions between compounds and their respective targets through molecular docking. From the current reports, HAEO can be used for development of natural drugs in near future.<\/jats:p>","DOI":"10.1002\/cbdv.202402254","type":"journal-article","created":{"date-parts":[[2024,11,27]],"date-time":"2024-11-27T13:34:28Z","timestamp":1732714468000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Assessment of Bioactivity of <i>Homalomena aromatica<\/i> Essential Oil From Northeast India Through Combinative In Vitro and In Silico Approaches"],"prefix":"10.1002","volume":"22","author":[{"given":"Tanmita","family":"Gupta","sequence":"first","affiliation":[{"name":"Agro\u2010Technology and Rural Development Division CSIR\u2010North East Institute of Science and Technology (NEIST)  Jorhat Assam India"},{"name":"AcSIR\u2010Academy of Scientific and Innovative Research  Ghaziabad Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prajisha","family":"Jayaprakash","sequence":"additional","affiliation":[{"name":"Agro\u2010Technology and Rural Development Division CSIR\u2010North East Institute of Science and Technology (NEIST)  Jorhat Assam India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raghu","family":"Tamang","sequence":"additional","affiliation":[{"name":"Agro\u2010Technology and Rural Development Division CSIR\u2010North East Institute of Science and Technology (NEIST)  Jorhat Assam India"},{"name":"AcSIR\u2010Academy of Scientific and Innovative Research  Ghaziabad Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neelav","family":"Sarma","sequence":"additional","affiliation":[{"name":"Agro\u2010Technology and Rural Development Division CSIR\u2010North East Institute of Science and Technology (NEIST)  Jorhat Assam India"},{"name":"AcSIR\u2010Academy of Scientific and Innovative Research  Ghaziabad Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Twahira","family":"Begum","sequence":"additional","affiliation":[{"name":"Agro\u2010Technology and Rural Development Division CSIR\u2010North East Institute of Science and Technology (NEIST)  Jorhat Assam India"},{"name":"AcSIR\u2010Academy of Scientific and Innovative Research  Ghaziabad Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6995-3377","authenticated-orcid":false,"given":"Mohan","family":"Lal","sequence":"additional","affiliation":[{"name":"Agro\u2010Technology and Rural Development Division CSIR\u2010North East Institute of Science and Technology (NEIST)  Jorhat Assam India"},{"name":"AcSIR\u2010Academy of Scientific and Innovative Research  Ghaziabad Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,12,9]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/0972060X.2021.1995510"},{"issue":"1","key":"e_1_2_9_3_1","first-page":"15","article-title":"Evaluation of Preliminary Phytochemical and In Vitro Antioxidant Activity of Homalomena aromatica (Tuber)","volume":"1","author":"Kumari M.","year":"2021","journal-title":"Journal of Applied Pharmacognosy and Phytochemistry"},{"key":"e_1_2_9_4_1","first-page":"101","article-title":"Indegenous Species Homalomena aromatica Schott of Tripura, India","volume":"2","author":"Debbarma R.","year":"2021","journal-title":"International Journal of Research"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.14719\/pst.2019.6.2.435"},{"issue":"1","key":"e_1_2_9_6_1","first-page":"18","article-title":"Phytochemical and Ethnopharmacological Overview of Endangered Homalomena aromatica Schott: An Aromatic Medicinal Herb of Northeast India","volume":"8","author":"Kehie M.","year":"2017","journal-title":"Indian Journal of Natural Products and Resources"},{"issue":"2","key":"e_1_2_9_7_1","first-page":"96","article-title":"Homalomena aromatica: An Ethnomedicinal Plant can be a Potential Source of Antimicrobial Drug Development","volume":"2","author":"Shukla A. 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