{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T23:02:17Z","timestamp":1787785337927,"version":"build-2784847793"},"reference-count":40,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T00:00:00Z","timestamp":1736121600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T00:00:00Z","timestamp":1736121600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study investigates the antimicrobial and anti\u2010inflammatory activities of fractions from the stem extract of\n                    <jats:italic>Bauhinia pulchella<\/jats:italic>\n                    Benth. The ethanolic extract was fractionated using column adsorption chromatography, resulting in a hexane fraction (J1) and a dichloromethane fraction (J2), both rich in terpenes and steroids. Characterization was performed using gas chromatography coupled with mass spectrometry (GC\u2010MS). Antimicrobial activity was assessed through minimal lethal concentrations (MLC) and minimal inhibitory concentrations (MIC), and the checkerboard method was employed to evaluate the synergistic effect. For anti\u2010inflammatory activity, an in vitro model using murine macrophage cell culture (RAW 264.7) was employed to measure the levels of nitric oxide (NO), TGF\u2010\u03b2, and TNF\u2010\u03b1. The results demonstrated antimicrobial activity against\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    ATCC 10226,\n                    <jats:italic>S. cholerasuis<\/jats:italic>\n                    ATCC 14028, the genus\n                    <jats:italic>Candida<\/jats:italic>\n                    ,\n                    <jats:italic>S. epidermidis<\/jats:italic>\n                    ATCC 12228, and\n                    <jats:italic>S. aureus<\/jats:italic>\n                    ATCC 25923. Synergism was observed between the fractions and cephalexin, norfloxacin, gentamicin, and polymyxin. The use of fractions J1 (125, 62.5, and 31.25\u00a0\u00b5g\/mL) and J2 (62.5\u00a0\u00b5g\/mL) resulted in a significant reduction in TNF\u2010\u03b1 levels. For NO and TGF\u2010\u03b2, both J1 (125, 62.5, and 31.25\u00a0\u00b5g\/mL) and J2 (62.5, 31.25, and 15.625\u00a0\u00b5g\/mL) fractions showed significant reductions in these inflammatory markers.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202402295","type":"journal-article","created":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T08:10:57Z","timestamp":1734423057000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Antimicrobial and Anti\u2010Inflammatory Activity of\n                    <i>Bauhinia Pulchella<\/i>\n                    Extract Fractions"],"prefix":"10.1002","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9229-6352","authenticated-orcid":false,"given":"Jarbas","family":"Lima de Carvalho","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Sciences Federal University of Pernambuco, Cidade Universit\u00e1ria  Recife Pernambuco 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Pharmacy Federal University of Cear\u00e1, Campus do Porangabussu  Fortaleza Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro Igor","family":"de Oliveira Pereira","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Federal University of Cear\u00e1, Campus do Porangabussu  Fortaleza Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raphael","family":"de Oliveira Rodrigues","sequence":"additional","affiliation":[{"name":"Department of Clinical and Toxicological Analysis Federal University of Cear\u00e1, Campus do Porangabussu  Fortaleza Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana Navarro","family":"Ueda Yaochite","sequence":"additional","affiliation":[{"name":"Department of Clinical and Toxicological Analysis Federal University of Cear\u00e1, Campus do Porangabussu  Fortaleza Cear\u00e1 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