{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:56:38Z","timestamp":1785891398814,"version":"3.56.0"},"reference-count":20,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,5,10]],"date-time":"2025-05-10T00:00:00Z","timestamp":1746835200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,5,10]],"date-time":"2025-05-10T00:00:00Z","timestamp":1746835200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    One new pyrazine derivative, 4\u2010[(8\u2032\u2010hydroxypyrazinyl)\u20102\u2032\u2010yl]\u20101,3,3\u2010butanetriol (\n                    <jats:bold>1<\/jats:bold>\n                    ), along with five known compounds (\n                    <jats:bold>2\u20136<\/jats:bold>\n                    ) was isolated from Zingiberis Rhizoma Praeparatum (ZRP). Their structures were elucidated by one\u2010dimensional (1D), two\u2010dimensional nuclear magnetic resonance (2D\u2010NMR), high\u2010resolution electrospray ionization mass spectrometry spectroscopic data analysis, quantum chemical calculations of NMR Shifts (DP4+), and electronic circular dichroism calculation. Compounds\n                    <jats:bold>1<\/jats:bold>\n                    ,\n                    <jats:bold>2<\/jats:bold>\n                    , and\n                    <jats:bold>5<\/jats:bold>\n                    demonstrated the protective effect on the hydrogen peroxide\u2010damaged human umbilical vein endothelial cells within the safe concentration range. Based on the results, those isolates exert their antioxidant effects by regulating the intracellular antioxidant enzymes superoxide dismutase, glutathione peroxidase, L\u2010lactic dehydrogenase activity, malonaldehyde content, and reactive oxygen species expression. Most noteworthy is the mentioned substances may secret vascular endothelial growth factors to promote the formation of blood tube generation. The results indicated that pyrazines and morpholines may be the candidate compositions of ZRP for the treatment of hemorrhagic disorders.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202402404","type":"journal-article","created":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T02:55:23Z","timestamp":1745895323000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Pyrazine Derivatives and Spiro Alkaloids From Zingiberis Rhizoma Praeparatum and Their Protective Effects on Hydrogen Peroxide\u2010induced Human Umbilical Vein Endothelial Cells"],"prefix":"10.1002","volume":"22","author":[{"given":"Qian","family":"Zhu","sequence":"first","affiliation":[{"name":"School of Pharmacy Anhui University of Chinese Medicine  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan\u2010Ru","family":"Li","sequence":"additional","affiliation":[{"name":"School of Pharmacy Anhui University of Chinese Medicine  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren\u2010Zhong","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmacy Anhui University of Chinese Medicine  Hefei China"},{"name":"Anhui Province Key Laboratory of Bioactive Natural Products  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fu\u2010cai","family":"Ren","sequence":"additional","affiliation":[{"name":"School of Pharmacy Anhui Medical University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wu\u2010Xi","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Pharmacy Anhui University of Chinese Medicine  Hefei China"},{"name":"Anhui Province Key Laboratory of Bioactive Natural Products  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong\u2010Su","family":"Zhao","sequence":"additional","affiliation":[{"name":"School of Pharmacy Anhui University of Chinese Medicine  Hefei China"},{"name":"Anhui Province Key Laboratory of New Manufacturing Technology for Traditional Chinese Medicine Decoction Pieces  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"De\u2010Ling","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Pharmacy Anhui University of Chinese Medicine  Hefei China"},{"name":"Anhui Province Key Laboratory of New Manufacturing Technology for Traditional Chinese Medicine Decoction Pieces  Hefei China"},{"name":"Anhui Province Key Laboratory of Bioactive Natural Products  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5956-1556","authenticated-orcid":false,"given":"Feng\u2010Qing","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Pharmacy Anhui University of Chinese Medicine  Hefei China"},{"name":"Anhui Province Key Laboratory of New Manufacturing Technology for Traditional Chinese Medicine Decoction Pieces  Hefei China"},{"name":"Anhui Province Key Laboratory of Bioactive Natural Products  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5,10]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"2118","article-title":"Status of Studying Natural Antioxidant in Ginger","volume":"8","author":"Huang X. 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