{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T23:02:09Z","timestamp":1772060529341,"version":"3.50.1"},"reference-count":72,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Kidney vetch (<jats:italic>Anthyllis vulneraria<\/jats:italic> L.) is an old medicinal plant mostly known for ethnomedicine. Nowadays, its herbal parts are only scarcely used in phytotherapy, whereas medicinal applications of roots have not been reported so far. In this study, we investigated ethyl acetate (EtOAc) extracts obtained by solvent partition of an acetone\/water crude extract of <jats:italic>A. vulneraria<\/jats:italic> roots. Mainly prenylated chalcones, flavones, isoflavones, and cinnamic acid derivatives dominated the compound profile when the extracts were studied by HPLC\u2013ESI\u2013(HR)MS<jats:sup>n<\/jats:sup> and GC\u2013MS analysis. Two main chalcones (<jats:bold>23<\/jats:bold> and <jats:bold>24<\/jats:bold>) were isolated from the EtOAc extracts by several consecutive preparative chromatographic steps. Based on 1D\u2010 and 2D\u2010NMR data <jats:bold>24<\/jats:bold> was assigned to the known compound kanzonol C, while <jats:bold>23<\/jats:bold> was identified as a novel chalcone. Moreover, antiproliferative activities of the EtOAc extract and of isolated chalcones were investigated in different liver, breast, and thyroid carcinoma cell lines. Interestingly, the EtOAc extract and <jats:bold>24<\/jats:bold> exhibited the most pronounced decrease of cell viability in the MDA\u2010MB468 breast carcinoma cell line with an IC<jats:sub>50<\/jats:sub> value of 5.97\u00a0\u00b5M. Two synthetic derivatives of <jats:bold>24<\/jats:bold> (hexahydro\u2010 and triacetyl kanzonol, <jats:bold>30<\/jats:bold>, <jats:bold>31<\/jats:bold>, respectively), <jats:bold>23<\/jats:bold> and isobavachalcone <jats:bold>18<\/jats:bold> were less active (IC<jats:sub>50<\/jats:sub>\u00a0&gt;\u00a011.7\u00a0\u00b5M). Thus, the prenylated chalcones detected in <jats:italic>A. vulneraria<\/jats:italic> roots appear to be promising lead structures for further antiproliferative studies.<\/jats:p>","DOI":"10.1002\/cbdv.202402634","type":"journal-article","created":{"date-parts":[[2025,2,18]],"date-time":"2025-02-18T11:33:36Z","timestamp":1739878416000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Prenylated Chalcones From Roots of Kidney Vetch (<i>Anthyllis vulneraria<\/i> L.) With Antiproliferative Activity"],"prefix":"10.1002","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8664-0911","authenticated-orcid":false,"given":"Peter","family":"Lorenz","sequence":"first","affiliation":[{"name":"Department of Analytical Development and Research WALA Heilmittel GmbH, Section Phytochemical Research  Bad Boll Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00fcrgen","family":"Conrad","sequence":"additional","affiliation":[{"name":"Institute of Chemistry, Bioorganic Chemistry (130b), University of Hohenheim  Stuttgart Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iris","family":"Klaiber","sequence":"additional","affiliation":[{"name":"Mass Spectrometry Unit, Core Facility, University of Hohenheim  Stuttgart Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lilo K.","family":"Mail\u00e4nder","sequence":"additional","affiliation":[{"name":"Department of Analytical Development and Research WALA Heilmittel GmbH, Section Phytochemical Research  Bad Boll Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khadijeh Nosrati","family":"Gazafroudi","sequence":"additional","affiliation":[{"name":"Department of Analytical Development and Research WALA Heilmittel GmbH, Section Phytochemical Research  Bad Boll Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Florian C.","family":"Stintzing","sequence":"additional","affiliation":[{"name":"Department of Analytical Development and Research WALA Heilmittel GmbH, Section Phytochemical Research  Bad Boll Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dietmar R.","family":"Kammerer","sequence":"additional","affiliation":[{"name":"Department of Analytical Development and Research WALA Heilmittel GmbH, Section Phytochemical Research  Bad Boll Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Celina","family":"Ablinger","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Biology and Clinical Pharmacy Institute of Pharmacy, Paracelsus Medical University  Salzburg Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petra","family":"Huber\u2010Cantonati","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Biology and Clinical Pharmacy Institute of Pharmacy, Paracelsus Medical University  Salzburg 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