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A total of 17 quinoline\u2010Schiff bases were synthesized with 56%\u201386% yields and characterized by using\n                    <jats:sup>1<\/jats:sup>\n                    H NMR,\n                    <jats:sup>13<\/jats:sup>\n                    C NMR, and high\u2010resolution mass spectra (HRMS) as potent anti\u2010mycobacterial agents with\n                    <jats:italic>Mtb<\/jats:italic>\n                    DNA gyrase inhibitory activity. Among all, compound\n                    <jats:bold>7f<\/jats:bold>\n                    displayed a significantly potent broad\u2010spectrum antitubercular and antimicrobial activity against most of the tested strains of bacteria and fungi, with minimum inhibitory concentration (MIC) values in the range of 1.95\u201315.62\u00a0\u00b5g\/mL. Seven quinoline\u2010Schiff bases (\n                    <jats:bold>7a<\/jats:bold>\n                    ,\n                    <jats:bold>7b<\/jats:bold>\n                    ,\n                    <jats:bold>7d<\/jats:bold>\n                    ,\n                    <jats:bold>7g<\/jats:bold>\n                    ,\n                    <jats:bold>7o<\/jats:bold>\n                    ,\n                    <jats:bold>7p<\/jats:bold>\n                    , and\n                    <jats:bold>7q<\/jats:bold>\n                    ) displayed a key improvement in both drug\u2010sensitive and drug\u2010resistant strains with MIC range of 3.90\u201315.62\u00a0\u00b5g\/mL, respectively. A subsequent in vitro study evaluated the most promising compounds (\n                    <jats:bold>7a<\/jats:bold>\n                    ,\n                    <jats:bold>7d<\/jats:bold>\n                    ,\n                    <jats:bold>7f<\/jats:bold>\n                    ,\n                    <jats:bold>7p<\/jats:bold>\n                    , and\n                    <jats:bold>7q<\/jats:bold>\n                    ) against\n                    <jats:italic>Mtb<\/jats:italic>\n                    DNA gyrase. Among these, compounds\n                    <jats:bold>7a<\/jats:bold>\n                    ,\n                    <jats:bold>7d<\/jats:bold>\n                    , and\n                    <jats:bold>7f<\/jats:bold>\n                    exhibited the highest activity, with IC\n                    <jats:sub>50<\/jats:sub>\n                    values of 1.98, 1.26, and 3.96\u00a0\u00b5M, respectively. Molecular docking studies revealed strong binding affinities of the synthesized derivatives with the\n                    <jats:italic>Mtb<\/jats:italic>\n                    DNA gyrase enzyme, highlighting key hydrogen and carbon\u2013hydrogen bond interactions with Tyr21, Gly84, Asn85, Asp110, Gln125, and pi\u2013pi and pi\u2010alkyl interactions with Arg62, Phe116, Pro118, Ala146, and Val147. Furthermore, ADMET profiling of the most effective derivatives\n                    <jats:bold>7a<\/jats:bold>\n                    ,\n                    <jats:bold>7d<\/jats:bold>\n                    , and\n                    <jats:bold>7f<\/jats:bold>\n                    demonstrated comparable bioavailability scores (0.55) with varied lipophilicity (MLOGP: 2.90\u20133.39) and lower TPSA compared to ciprofloxacin (CFX). These comprehensive analyses and properties suggest favorable drug\u2010likeness with potential for further optimization.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202402644","type":"journal-article","created":{"date-parts":[[2025,1,29]],"date-time":"2025-01-29T01:09:47Z","timestamp":1738112987000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Design and Evaluation of Quinoline\u2010Schiff Bases Targeting\n                    <i>Mtb<\/i>\n                    DNA Gyrase: In Vitro and Computational Approaches"],"prefix":"10.1002","volume":"22","author":[{"given":"Baji Baba","family":"Shaik","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Chemistry, Discipline of Pharmaceutical Sciences, College of Health Sciences University of KwaZulu\u2010Natal, Westville Campus  Durban South 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