{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T18:36:02Z","timestamp":1792175762527,"version":"4.4.0"},"reference-count":80,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,1,30]],"date-time":"2025-01-30T00:00:00Z","timestamp":1738195200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,1,30]],"date-time":"2025-01-30T00:00:00Z","timestamp":1738195200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The\n                    <jats:italic>Lentinus edodes<\/jats:italic>\n                    polysaccharide (LEP) was extracted with a new subcritical water extraction (SWE) enhanced with deep eutectic solvent (DES) method and then purified with a DEAE\u201052 cellulose column and a Sephadex G\u2010100 column. Two purified polysaccharides (LEP1 and LEP2) were obtained, and their structure, antioxidant activity, and immunomodulatory activity were analyzed. LEP1 and LEP2 were composed of mannose, glucose, and galactose with a molar ratio of 1:12.97:7.84 and 1:51.18:5.29, respectively. The molecular weights were 9.878\u00a0\u00d7\u00a010\n                    <jats:sup>4<\/jats:sup>\n                    and 1.976\u00a0\u00d7\u00a010\n                    <jats:sup>4<\/jats:sup>\n                    \u00a0Da, respectively. Interestingly, both LEP1 and LEP2 were mainly composed of \u21924)\u2010\u03b2\u2010\n                    <jats:sc>d<\/jats:sc>\n                    \u2010Glc\n                    <jats:italic>p<\/jats:italic>\n                    \u2010(1\u2192, \u21926)\u2010\u03b2\u2010\n                    <jats:sc>d<\/jats:sc>\n                    \u2010Glc\n                    <jats:italic>p<\/jats:italic>\n                    \u2010(1\u2192 and \u21926)\u2010\u03b1\u2010\n                    <jats:sc>d<\/jats:sc>\n                    \u2010Gal\n                    <jats:italic>p<\/jats:italic>\n                    \u2010(1\u2192 with different molar ratio. Besides, both LEP1 and LEP2 had strong DPPH free radical scavenging activity and Fe\n                    <jats:sup>2+<\/jats:sup>\n                    chelating capacity. Moreover, they could reduce the level of reactive oxygen species (ROS) and regulate the activities of malondialdehyde (MDA), catalase (CAT), and superoxide dismutase (SOD) in HepG2 cells, demonstrating strong antioxidant activity. Furthermore, both LEP1 and LEP2 could improve the phagocytic capacity, nitric oxide (NO) release, and the content of interleukin (IL)\u20106, IL\u20101\u03b2, and tumor necrosis factor\u2010\u03b1 (TNF\u2010\u03b1) in RAW264.7 cells, exhibiting significant immunostimulatory activity. It was worth noting that LEP2 exhibited stronger biological activities than LEP1. Therefore, the SWE enhanced with DES is an ideal method for extracting polysaccharides, which can be further applied to extract other polysaccharides.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202402658","type":"journal-article","created":{"date-parts":[[2025,1,18]],"date-time":"2025-01-18T09:43:29Z","timestamp":1737193409000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Isolation, Purification, and Characterization of\n                    <i>Lentinus edodes<\/i>\n                    Polysaccharides Extracted With Subcritical Water Enhanced With Deep Eutectic Solvent"],"prefix":"10.1002","volume":"22","author":[{"given":"Chaoting","family":"Wen","sequence":"first","affiliation":[{"name":"College of Food Science and Engineering Yangzhou University  Yangzhou 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